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Artificial cultivation method of boletus rubellus krombh

A technology of artificial cultivation and boletus, applied in cultivation, mushroom cultivation, plant cultivation, etc., can solve the problems of no artificial cultivation, and achieve the effect of low cost, simple raw materials, and short cultivation period

Inactive Publication Date: 2017-11-07
FOSHAN TUIQI AGRI RES INST GENERAL PARTNERSHIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rich in ergosterol, ergosterol peroxide and ergoster-4,6,8(14),22-tetraen-3-one, whose structure types belong to pyrazines, phenols and steroids respectively, with antibacterial , anti-inflammatory, insecticidal, anti-tumor, anti-complement, immunosuppressive, anti-influenza virus, anti-insect and other biological activities, with high medicinal value, it is the preferred raw material for extracting medicinal bioactive substances, but there are only a few information about its form and Reports on chemical components, but no research on artificial cultivation

Method used

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  • Artificial cultivation method of boletus rubellus krombh
  • Artificial cultivation method of boletus rubellus krombh

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Culture substrate preparation, described culture substrate contains the raw material of following weight ratio: 40 parts of chestnut sawdust, 20 parts of corn flour, 20 parts of yellow sandy soil, MgSO 4 ·7H 2 O 0.6 parts, CaCO 3 0.8 parts, KH 2 PO 4 0.5 parts, 6 parts of Ural licorice extract; the pH of the culture substrate is 4.8;

[0022] (2) Inoculation and bacterium cultivation, the boletus haemorrhoids bacterial classification is inoculated in the sterilized culture medium of step (1), keep temperature 20 DEG C, humidity 75%, CO 2 The concentration is 900PPm, and cultured in shading until the culture medium is covered with hyphae;

[0023] (3) Cover the soil, vibrate and mix the yellow sandy soil and vegetable garden soil in a ratio of 1:2 on the shaker, and spray water at the same time to make the soil moisture content reach 70%, stack it for 8 days and cover it on the substrate covered with mycelia in step (2) ;

[0024] (4) Cultivate mycelia in the...

Embodiment 2

[0027] (1) Culture substrate preparation, described culture substrate contains the raw material of following weight ratio: 50 parts of chestnut sawdust, 25 parts of corn flour, 25 parts of yellow sandy soil, MgSO 4 ·7H 2 O 1.1 parts, CaCO 3 1.1 parts, KH 2 PO 4 0.75 parts, 8 parts of Glycyrrhiza glabra extract; the pH of the culture substrate is 5.3;

[0028] (2) inoculation, bacterium cultivation, the boletus haemorrhoids bacterial classification is inoculated in the sterilized culture medium of step (1), keep temperature 22.5 ℃, humidity 80%, CO 2 Concentration 1050PPm, cultured in shading until the culture medium is covered with hyphae;

[0029] (3) Covering soil, vibrate and mix yellow sand soil and vegetable garden soil in a ratio of 1:3 on a shaker, add 20% banana peel pulp, mix evenly, and spray water at the same time to make the soil moisture content reach 75%, and cover it after stacking for 10 days Step (2) on the substrate covered with hyphae;

[0030] (4) C...

Embodiment 3

[0033] (1) Culture substrate preparation, described culture substrate contains the raw material of following weight ratio: 60 parts of chestnut sawdust, 30 parts of corn flour, 30 parts of yellow sandy soil, MgSO 4 ·7H 2 O 1.6 parts, CaCO 3 1.4 parts, KH 2 PO 4 1 part, 10 parts of Ural Glycyrrhiza extract, Glycyrrhiza glabra extract or Glycyrrhiza inflate extract; pH of the culture substrate is 5.8;

[0034] (2) inoculation, bacterium cultivation, the boletus haemorrhoids strain is inoculated in the sterilized culture medium of step (1), keep temperature 25 ℃, humidity 85%, CO 2 Concentration of 1200PPm, shading culture until the culture medium is covered with hyphae;

[0035] (3) Covering soil, vibrate and mix yellow sandy soil and vegetable garden soil in a ratio of 1:4 on a shaker, add 25% banana peel slurry, mix evenly, and spray water at the same time to make the soil moisture content reach 80%, pile it up for 12 days and cover it Step (2) on the substrate covered ...

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Abstract

The invention discloses an artificial cultivation method of boletus rubellus krombh. The method comprises the steps of (1) preparation of culture medium, wherein the culture medium comprises, by weight, 40-60 parts of Chinese chestnut wood flour, 20-30 parts of corn powder, 20-30 parts of sand soil, 0.6-1.6 parts of MgSO4.7H2O, 0.8-1.4 parts of CaCO3, 0.5-1 part of KH2PO4 and 6-10 parts of licorice extract; the pH of the culture medium is 4.8-5.8; (2) inoculation and bacterial culturing; (3) soil covering; (4) overbarden mycelium culture; (5) fruiting culture. The artificial cultivation method of the boletus rubellus krombh has the advantages that the artificial cultivation method of the boletus rubellus krombh is provided for the first time, and the cultivation period is short; raw materials are simple, and the cost is low; the fatty acid content of cultured boletus rubellus krombh is high; the method lays the foundation for scaled cultivation of the boletus rubellus krombh; the method is expected to provide sufficient raw materials for medicinal bioactive substances.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for artificially cultivating boletus sanguineum. Background technique [0002] Blood-red boletus (Boletus rubellus Krombh.) has another name called vermilion boletus, is Basidiomycetes, Agaricsles (Agaricsles), Boletaceae (Boletaceae), a kind of edible fungus of Boletus (Boletus). Mainly distributed in my country's Yunnan, Sichuan, Jilin, Liaoning and other places. The fruiting body of the fungus is medium and large, the cap is oblate hemispherical to slightly flattened, blood red to purple-brown, with fine hairs or cracks, 4-10cm in diameter, and the initial cap margin rolls inward. The flesh of the bacteria is white to yellowish, reddish near the epidermis, and the wound turns blue-green, with a mild taste. Bacterial tubes are straight or slightly elongated at the stipe, become dark yellow when old, and turn blue-green when wounded, with...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00C05F15/00C05F17/00
CPCA01G18/00C05B7/00C05F5/002C05F17/00C05G3/00C05D3/02C05D5/00C05D9/00C05F11/00C05F11/08Y02W30/40
Inventor 曹丽萍
Owner FOSHAN TUIQI AGRI RES INST GENERAL PARTNERSHIP
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