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Cas9-mediated tomato gene editing vector and application thereof

A tomato and gene technology, applied in the field of genetic engineering, can solve the problems of long shelf life of genetically modified products, display the mainstream characteristics of genetically modified technology, etc., and achieve the effect of improving storage tolerance

Inactive Publication Date: 2017-11-03
HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

There are already storable tomato varieties on the market, which are basically bred through traditional cross-breeding methods, but the storability of these varieties can only be basically qualified, and the storage time of transgenic products will be relatively long, and The product can effectively improve the bacterial infection after fruit picking, which is a major problem in storage and transportation
Although the debate on genetically modified technology has never stopped since its birth, the speed of technological development is changing with each passing day. With the rapid development of its bio-economy, global genetically modified crops continue to grow year by year, all of which show the importance of genetically modified technology in breeding. mainstream features

Method used

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  • Cas9-mediated tomato gene editing vector and application thereof
  • Cas9-mediated tomato gene editing vector and application thereof
  • Cas9-mediated tomato gene editing vector and application thereof

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Experimental program
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Embodiment 、1）

[0025] Embodiment, 1), material

[0026] The direct source of tomato M82 seeds was cultivated and preserved by Horticultural Crops Research Institute of Xinjiang Academy of Agricultural Sciences. The original source was introduced by Wang Boke from American Tomato Genetics Center in September 2008.

[0027] The high-fidelity polymerase, DNA recovery kit and cloning vector used in PCR were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Restriction enzymes and ligases were purchased from TaKaRa Company, PCR primer synthesis and fragment sequencing were commissioned by Sangon Bioengineering (Shanghai) Co., Ltd.

[0028] 2) Construction of expression vector

[0029] The spCas9 vector was obtained from Gao Caixia, a researcher at the Chinese Academy of Sciences. This vector is an open vector. General researchers can import it through the online plasmid library (http: / / www.addgene.org / ), and the designed target can be directly synthesized by Beijing Kangwei Biotechnology...

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Abstract

The invention belongs to the field of genetic engineering technology, and specifically discloses a Cas9-mediated tomato gene editing vector and application thereof. A Cas9 gene is introduced into a tomato M82 by constructing a CRISPR / Cas9 tomato plant expression vector, and gene editing is carried out on the DFD gene of the tomato. The invention provides a method for constructing a storage-endurance transgenic tomato, the new storage-endurance tomato product is obtained, so that the storage endurance of the tomato fruit is improved, and besides, the growth and other qualities of plant are not influenced, and the method can compensate for shortcomings of former research programs.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically discloses a Cas9-mediated tomato gene editing vector and an application thereof. Background technique [0002] The limitation of storage and transportation time and shelf life is the main problem faced by horticultural products. Extending the storage and transportation time of vegetables and fruits will bring huge benefits to all links in the supply chain, from farmers to wholesalers to retailers and final consumers, especially It is the status quo of Chinese fresh products whose production and distribution channel efficiency is not high. Studies have shown that the loss rate of fresh vegetables in the entire supply chain is as high as 20%-30%. It can be improved through genetically modified technology and has a huge market potential. Tomato is an extremely widely cultivated vegetable in the world, and it is also one of the main cultivated vegetables in my country. ...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N9/22A01H5/00
CPCC12N15/8213C12N9/22C12N15/8261
Inventor 李宁王柏柯唐亚萍杨生保帕提古丽·艾斯木托拉杨涛余庆辉
Owner HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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