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A collagen extraction process based on small yellow croaker scales

A technology of fish scale collagen and collagen, which is applied in the field of collagen extraction technology, and can solve the problems of unresearched collagen extraction technology

Active Publication Date: 2018-09-04
华夏仁心(北京)医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As an important aquatic product in eastern my country, small yellow croakers appear on the dining tables of citizens in large quantities, but there is no research on the collagen extraction process of small yellow croaker scales

Method used

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  • A collagen extraction process based on small yellow croaker scales
  • A collagen extraction process based on small yellow croaker scales
  • A collagen extraction process based on small yellow croaker scales

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Embodiment Construction

[0031] The present invention is specifically described below by the embodiment, only for further illustrating the present invention, can not be interpreted as the limitation of protection scope of the present invention, the technical engineer of this field can make some non-essential improvements and improvements to the present invention according to the content of the above-mentioned invention Adjustment. 1 Materials and methods

[0032] 1.1 Materials and sources

[0033] Small yellow croaker scales were provided by Zhejiang Wenzhou Xianghai Food Co., Ltd.

[0034] 1.2 The main instruments and equipment of the test

[0035] HHS type electric heating constant temperature water bath (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory);

[0036] LT1002E electronic balance (Changshu Tianliang Instrument Co., Ltd.);

[0037] AR2140 electronic balance (Mettler Toledo Instruments Shanghai Co., Ltd.);

[0038] BCD-226SDCZ refrigerator (Haier Group);

[0039] DHG-9246...

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Abstract

The invention discloses a collagen extraction process based on fish scales of small yellow croakers. The process comprises the following steps: (1) pre-treatment: cleanly washing the fish scales of the small yellow croakers, and soaking the fish scales with 5 percent of sodium hydroxide for 22 hours; washing the soaked fish scales with tap water until washing liquid is neutral, and soaking with 10 percent of citric acid for 2 hours; then washing with the water again until the washing liquid is neutral, putting in a 40 DEG C electric constant temperature drying oven for carrying out constant temperature treatment for 8 hours, and finally putting in a pulverizing machine to be pulverized into powder to obtain fish scale powder of the small yellow croakers; (2) extracting the fish scale powder of the small yellow croakers in a constant-temperature water bath pot of 66.5 DEG C for 246 minutes according to a feed-liquid ratio of mass volume ratio of 1 to 13 to obtain collagen extraction liquid; (3) carrying out separation to obtain collagen crude extracts of the fish scales of small yellow croakers. The collagen extraction process disclosed by the invention has the advantages that an original protein structure is substantially preserved and the extraction purity of the collagen is high.

Description

technical field [0001] The invention relates to a biological extraction process, in particular to a collagen extraction process based on small yellow croaker scales. Background technique [0002] In recent years, collagen has become one of the research hotspots at home and abroad because of its unique biological characteristics, which are not only related to cell differentiation, proliferation and wound healing, but also related to biological immune characteristics. The collagen content in aquatic products accounts for 25-30% of the total protein content of fish, mainly distributed in fish scales and skins, and the crude collagen in fish scales and fish skins accounts for about 80%. Throw away this part as leftovers; at the same time, most low-value fish and shrimp are also rich in protein, so the development and utilization of protein sources has become the main way for the deep utilization of low-value fish and shrimp and aquatic product processing leftovers. [0003] Stu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/30C12P21/06
CPCC07K14/78C12P21/06
Inventor 苏凤贤张百刚杨意杜琨邹盈
Owner 华夏仁心(北京)医学研究院有限公司
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