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TiO2 immobilized enzyme reactor and application thereof

A technology for immobilizing enzymes and reactors, applied in enzyme production/bioreactors, immobilizing on or in inorganic carriers, etc., can solve the problems of dye toxicity, difficult to degrade, expensive equipment and materials, secondary pollution, etc. The effect of low cost, simple preparation process, and efficient catalytic degradation ability

Active Publication Date: 2017-10-27
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods can effectively remove the color of dye wastewater, but the toxicity of the dye itself is difficult to degrade. Excessive substances may also cause secondary pollution

Method used

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  • TiO2 immobilized enzyme reactor and application thereof
  • TiO2 immobilized enzyme reactor and application thereof
  • TiO2 immobilized enzyme reactor and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 1. Put 80mg TiO 2 and 9.5 mg of dopamine hydrochloride were added to 50 mL of ultrapure water, and 102 mg of hexamethylenetetramine was added, stirred in a vortex for 30 seconds, incubated at 90°C for 3 hours, centrifuged at 10,000 rpm for 2 minutes to remove the supernatant, and the remaining After the solid was washed with water and ethanol, the dopamine-modified TiO 2 .

[0020] 2. Add 50mg of dopamine-modified TiO 2 Add it into 18mL of Tris-HCl buffer solution with pH=7.0, ultrasonically disperse for 15 minutes, then add 2mL of glutaraldehyde aqueous solution with a volume fraction of 50%, stir at room temperature for 2 hours, and centrifuge to separate the obtained solid product into 1.6mL containing 0.2 In the PBS buffer solution of mmol / L chloroperoxidase pH=4.5, stir at 4°C for 12 hours, centrifuge, discard the supernatant, and dry the remaining solid at 30°C to obtain TiO 2 Immobilized Chloroperoxidase Reactor.

Embodiment 2

[0022] 1. Put 80mg TiO 2 and 9.5 mg of dopamine hydrochloride were added to 50 mL of ultrapure water, and 102 mg of hexamethylenetetramine was added, stirred in a vortex for 30 seconds, incubated at 90°C for 3 hours, centrifuged at 10,000 rpm for 2 minutes to remove the supernatant, and the remaining After the solid was washed with water and ethanol, the dopamine-modified TiO 2 .

[0023] 2. Add 50mg of dopamine-modified TiO 2 Add it into 18mL of Tris-HCl buffer solution with pH=7.0, ultrasonically disperse for 15 minutes, then add 2mL of glutaraldehyde aqueous solution with a volume fraction of 50%, stir at room temperature for 2 hours, and centrifuge to separate the obtained solid product into 1.6mL containing 0.5 mg / mL horseradish peroxidase in PBS buffer at pH = 7.0, stirred at 4°C for 12 hours, centrifuged, discarded the supernatant, and dried the remaining solid at 30°C to obtain TiO 2 Immobilized horseradish peroxidase reactor.

Embodiment 3

[0025] The TiO that embodiment 1 prepares 2 The application of immobilized chloroperoxidase reactor in catalytic degradation of crystal violet, the specific use method is:

[0026] Weigh 10mg TiO 2 Add the immobilized chloroperoxidase reactor to 1.5mL of PBS buffer solution containing 0.5mmol / L crystal violet at pH=2.75, then add 30μL of 0.1mol / L hydrogen peroxide, react at room temperature, and centrifuge after the reaction , take the supernatant to measure its absorbance value. Calculate the degradation rate of the dye according to the following formula:

[0027] η=(A 0 -A t ) / A 0 ×100%

[0028] In the formula, η is the degradation rate, A 0 is the initial absorbance value of the dye, A t is the absorbance value of the dye at time t. The test results show that it only takes 1-2 minutes to degrade more than 90% of the crystal violet in the first 10 degrading processes. like figure 1 Shown, reacted at room temperature for 10min, the enzyme reactor relative to the do...

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Abstract

The invention discloses a TiO2 immobilized enzyme reactor and application thereof. By using dopamine-modified TiO2 as a carrier and glutaraldehyde as a cross-linking agent, chloroperoxidase or horseradish peroxidase is immobilized on a surface of the carrier through a covalent bonding action to prepare the TiO2 immobilized enzyme reactor. A preparation process is simple and low in cost; the obtained enzyme reactor has high-efficient catalytic degradation capability on dyes (crystal violet, crocein orange G, alizarin red and the like) and 2,4-dichlorophenol, the degradation rate can reach nearly 100 percent within extremely short time, and a repeated use effect is good.

Description

technical field [0001] The invention belongs to the technical field of dye wastewater and domestic sewage treatment, and specifically relates to an immobilized enzyme reactor and its application in the rapid degradation of dyes and the efficient degradation of 2,4-dichlorophenol in domestic sewage. Background technique [0002] my country's annual dye export volume is about 300,000 tons, imports are 50,000 tons, and dyes used in fabric printing and dyeing are about 200,000 tons. It is mainly composed of azo and anthraquinones, and has good application performance and excellent fastness performance. The amount of wastewater discharged by the dye industry in my country is huge. According to statistics, about 1% to 2% of dyes are used in the dye production process, and about 10% of the dyes will be discharged into the natural environment with the wastewater during the dyeing process, which is harmful to the environment. posed a serious threat. The decolorization and degradatio...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12N11/14A62D3/02A62D101/28
CPCA62D3/02A62D2101/28C12M21/18C12N11/14
Inventor 蒋育澄程涵萍胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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