Method of extracting lagedium sibiricum type bitter principle from chicories

Abstract

The invention discloses a method of extracting lagedium sibiricum type bitter principle from chicories. The method comprises the following steps: crushing chicory roots, performing extracting with water; performing filtering, and performing reduced pressure concentration to obtain a total extract; dispersing the total extract into water; performing extraction with ethyl acetate; performing reduced pressure distillation to obtain an ethyl acetate extract; and dissolving the ethyl acetate extract obtained in the second step and performing gradient elution separation, wherein a mobile phase A is ethyl acetate, a mobile phase B is formic acid, a mobile phase C is n-butyl alcohol and the elution condition comprises 0-4 min, 45% A, 50% B and 5% C; 4-10 min, 45%-40% A, 50%-55% B and 5% C; 10-14 min, 40%-30% A, 55% B and 5%-15% C; and 14-20 min, 30% A, 55% B and 15% C. The extracting method provided by the invention is convenient and feasible, and the purities of separated lactucin and lactucopicrin are both higher than 98.0%.

Description

Technical field [0001] The present invention relates to a separation method, in particular to a method for extracting bittersweet from chicory. Background technique [0002] Chicory is also known as European chicory or French cocory. It is called "bitter cabbage" in Japan. It is a plant of the genus Compositae with the same medicine and food. Studies have shown that chicory contains bitter substances that are not found in general vegetables, such as horse chestnut cortex, horse chestnut cortex glycosides, lyctophyllin and lyctobacter, etc. These bitter substances have antibacterial properties, improve digestion, clear liver and gallbladder, and relieve Wine and other effects. [0003] At present, the research on the chemical constituents of plants of the genus Cichorium mainly focuses on sesquiterpenoids. The sesquiterpenes in chicory as long as there are Lactucanum and Lactucanum, the molecular formula of Lactucanum is C 15 H 16 O 5 ,The molecular formula of Lactucae is C 23 H 22...

AI Technical Summary

Problems solved by technology

[0004] The Chinese invention patent with the authorized announcement number of CN102351824B provides a preparation method of olectuca and lentopicrin. In the above method, high-speed counter-current chromatography is used to separate the celtuce and celtuce, but it involves five kinds of The configuration and separation operation of the mixed solvent of the components, the overall separation process is slightly complicated