Method for promoting earlier blooming of chrysanthemums by utilizing grafting mediated RNAi (Ribonucleic Acid interfere) technology

A technology of early flowering and chrysanthemum, which is applied in the field of plant cultivation, can solve the problems of time-consuming and laborious, and achieve the effect of avoiding transgenic operations, objective production benefits, and good promotion and application value

Inactive Publication Date: 2017-10-20
HENAN UNIVERSITY
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Problems solved by technology

However, there are certain defects in the application of this patented technology, mainly: RNAi transgenic plants may naturally hybridize with chrysanthemums in the surrounding natural growth conditions through pollination, and then there is a risk of gene drift. In addition, this method regulates the flowering period of chrysanthemums. Every time a variety is regulated, a transgenic process must be carried out, which is time-consuming and laborious

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  • Method for promoting earlier blooming of chrysanthemums by utilizing grafting mediated RNAi (Ribonucleic Acid interfere) technology

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Embodiment

[0028] The method for promoting early flowering of chrysanthemums provided by grafting-mediated RNAi technology in this application, the specific operation process is briefly introduced as follows.

[0029] (1) Prepare the rootstock, taking the transgenic "Purple Fairy" chrysanthemum material after RNAi interference with the MET1 gene as an example. Due to the relatively limited raw materials, appropriate amplification and propagation were first carried out for standby. The base is: MS + 1.0 mg / L 6-BA + 0.15 mg / L NAA (pH = 5.8), and then use MS medium as the rooting medium for rooting, and the formula is MS + 3 % sucrose. The culture conditions are: temperature (25±1)°C, light intensity of 2000 Lux, and 12 light hours per day.

[0030] After the chrysanthemum seedlings take root, continue to cultivate for 1 to 2 months before carrying out conventional seedling hardening. When hardening the seedlings, transplant the rooted seedlings to the hole tray for hardening. The culture m...

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Abstract

The invention belongs to the technical field of plant cultivation, relates to a regulation and control method for a plant blooming stage and more specifically relates to a method for promoting earlier blooming of chrysanthemums by utilizing a grafting mediated RNAi (Ribonucleic Acid interfere) technology. The method comprises the following steps: carrying out rootstock preparation, carrying out scion selection, carrying out grafting operation and the like; and a rootstock is a transgenic chrysanthemum material with a methylation gene which is silenced by adopting the RNAi technology. According to the method provided by the invention, the transgenic chrysanthemum material is only used as the rootstock and is not used for blooming, so that bio-safety risks of genetic drift can be avoided relatively well; and furthermore, a grafting technology is applied so that the earlier blooming of different chrysanthemum varieties can be realized through the material of only one transgenic chrysanthemum variety, transgenic operation of each variety is avoided and the applicability is relatively good. According to the method for promoting the earlier blooming of the chrysanthemums, provided by the invention, the earlier blooming aim is realized relatively well on the basis of ensuring the normal growth of plants and stabilizing the quality of the chrysanthemums, so that the method has relatively good application values on cultivation of earlier blooming series of good varieties.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a method for regulating the florescence of plants, and more specifically, relates to a method for promoting early flowering of chrysanthemums through grafting-mediated RNAi technology. Background technique [0002] Chrysanthemum has a relatively long cultivation period due to its autumn flowering characteristics, which affects the application of chrysanthemum to a certain extent. For example, most chrysanthemum varieties bloom after the National Day, which affects their application in the National Day tourist season. Therefore, promoting the early flowering of chrysanthemums is of great significance for reducing cultivation costs. [0003] Studies on the regulation of chrysanthemum flowering have shown that short-day exposure and methylation inhibitors (5-azac, curcumin, decitabine, etc.) can advance the flowering of many varieties of chrysanthemum. The time can be adva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06A01H4/00
CPCA01H4/00A01H4/001
Inventor 王子成李忠爱李飞李帅磊李茫茫刘艳华范晓萱
Owner HENAN UNIVERSITY
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