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High-throughput, high-content and parallel imaging device and screening system for biological cell chips

A technology of biological cells and imaging devices, applied in the field of imaging, can solve the problems of inconsistency between resolution and sample parallelism, time difference (ten minutes to several hours, no parallel comparability, etc.), and achieve parallel imaging and screening, research and development. The effect of improving the level and maximizing the utilization rate

Active Publication Date: 2017-10-17
PEKING UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0009] (2) There is no parallel comparability between kinetic experiment samples
Through multiple scans and image stitching in each well, there is a significant time difference between wells (ranging from ten minutes to several hours), and it is impossible to analyze the kinetic changes of the sample (such as the drug triggering of the calcium ion signal of the cell) changes, dynamic changes in mitochondrial membrane potential) for simultaneous parallel observation
[0010] (3) Resolution and sample parallelism cannot be taken into consideration. It is necessary to reduce the resolution and increase the imaging speed of each field of view to reduce the time difference between holes, or sacrifice the comparability and time difference of imaging between holes to improve resolution.

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  • High-throughput, high-content and parallel imaging device and screening system for biological cell chips

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Embodiment Construction

[0030] In the drawings, the same or similar reference numerals are used to denote the same or similar elements or elements having the same or similar functions. Embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0031] In the description of the present invention, the terms "central", "longitudinal", "transverse", "front", "rear", "left", "right", "vertical", "horizontal", "top", " The orientation or positional relationship indicated by "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying the referred device or element Must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be construed as limiting the scope of the invention.

[0032] Such as Figure 1 to Figure...

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Abstract

The invention discloses a high-throughput, high-content and parallel imaging device and a screening system for biological cell chips. The high-throughput, high-content and parallel imaging device comprises a sample table, a light source assembly, a primary imaging assembly, a secondary imaging assembly and a detector. First imaging is integrally completely carried out on cell island array regions of the biological cell chips in first imaging planes by the primary imaging assembly in optical axis sharing imaging modes, and first formed images on the first imaging planes comprise first hole effective regions which are separately arranged in array forms; second imaging is completely carried out on the various first hole effective regions on detection planes of the detector by the secondary imaging assembly in light path splitting imaging modes, second formed images on the detection planes of the detector comprise second hole effective regions which are separately arranged in array forms, and gaps between every two adjacent second hole effective regions are determined by secondary preset amplification factors. The high-throughput, high-content and parallel imaging device and the screening system have the advantages that gapless imaging can be carried out on the various hole effective regions of the biological cell chips, the utilization rate of camera pixels can be maximized, and accordingly high-throughput, high-content and parallel imaging and screening can be carried out.

Description

technical field [0001] The invention relates to the field of imaging technology, in particular to a high-throughput, high-content, parallel imaging device and screening system for a biological cell chip. Background technique [0002] Explanation of terms: "High throughput" corresponds to "large field of view", "high content" corresponds to "high resolution", and "parallel" corresponds to "multi-hole synchronous" imaging. [0003] High-throughput and high-content screening technology refers to the simultaneous detection of the influence of samples on various aspects of cell morphology, growth, apoptosis, metabolic pathways and signal transduction under the premise of maintaining the integrity of cell structure and function, and to determine its biological activity and Potentially toxic. Compared with molecular-level screening such as proteins, cell-level screening eliminates the need for purification of the target protein, making the conformation and environment of the targe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34
CPCG01N33/5008
Inventor 毛珩
Owner PEKING UNIV
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