Treatment method of papermaking wastewater
A papermaking wastewater and treatment method technology, applied in the field of papermaking industry, can solve the problems of high cost, low recycling rate, and large residual amount of toxic and harmful substances, and achieve the effect of reducing COD and BOD values and realizing maximum utilization
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Embodiment 1
[0019] A method for treating papermaking wastewater, comprising the steps of:
[0020] (1) Cultivate microbial cells
[0021] Inoculate the mixed strains on the prepared medium, shake and culture at 28°C for 30 hours, and culture at 20°C for 20 hours, pour the culture solution into a centrifuge tube, and centrifuge at 4500 rpm for 8 minutes , remove the supernatant, wash the residue twice with PBS buffer, collect the microbial cells and store them at 5°C for later use. The content of each component of the medium is: beef extract 1.5g / L, glucose 1.0g / L, tryptone 4.5g / L, yeast powder 4.0g / L, sodium chloride 0.5g / L, and the balance is a universal carrier;
[0022] (2) Enhanced aerobic granular sludge
[0023] Disperse the cultured microbial cells in a glucose solution with a concentration of 5g / L according to the volume ratio of 1:1000, and spread them on the dry aerobic granular sludge. Rotate the culture for 20 hours, adjust the pH value of the papermaking wastewater to 6.8...
Embodiment 2
[0030] A method for treating papermaking wastewater, comprising the steps of:
[0031] (1) Cultivate microbial cells
[0032] Inoculate the mixed strains on the prepared medium, shake and culture at 30°C for 33 hours, and culture at 23°C for 22 hours, pour the culture solution into a centrifuge tube, and centrifuge at 4800 rpm for 9 minutes , remove the supernatant, wash the residue twice with PBS buffer, collect the microbial cells and store them at 6°C for later use. The content of each component of the medium is: beef extract 1.7g / L, glucose 1.2g / L, tryptone 5.0g / L, yeast powder 4.5g / L, sodium chloride 0.7g / L, and the balance is a universal carrier;
[0033] (2) Enhanced aerobic granular sludge
[0034] Disperse the cultured microbial cells in a glucose solution with a concentration of 6g / L according to the volume ratio of 1:1000, and spread them on the dry aerobic granular sludge. Rotate the culture for 22 hours, adjust the pH value of the papermaking wastewater to 6.9...
Embodiment 3
[0041] A method for treating papermaking wastewater, comprising the steps of:
[0042] (1) Cultivate microbial cells
[0043]Inoculate the mixed strains on the prepared culture medium, culture with shaking at 32°C for 36 hours, and culture at 25°C for 24 hours, pour the culture solution into a centrifuge tube, and centrifuge at 5000 rpm for 10 minutes , remove the supernatant, wash the residue with PBS buffer three times, collect the microbial cells and store them at 8°C for later use. The content of each component of the medium is: beef extract 2.0g / L, glucose 1.5g / L, tryptone 5.5g / L, yeast powder 5.0g / L, sodium chloride 1.0g / L, and the balance is a universal carrier;
[0044] (2) Enhanced aerobic granular sludge
[0045] Disperse the cultured microbial cells in a glucose solution with a concentration of 7g / L according to the volume ratio of 1:1000, and spread them on the dry aerobic granular sludge. Rotate culture for 24 hours, adjust the pH value of the papermaking wast...
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