Method for producing propionate mildew preventive through microbial fermentation and use thereof

A technology of a microbial fermentation method and a production method is applied in the field of the production of propionate antifungal agents by a microbial fermentation method. Simple process and the effect of reducing production costs

Inactive Publication Date: 2017-10-03
HEFEI HUAGAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production methods of calcium propionate and ammonium propionate are mostly multi-purpose chemical methods, but this method has obvious disadvantages, such as complex process flow, various equipment, high requirements for equipment and pipeline materials; harsh reaction conditions, high energy consumption , a big impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:

[0052] (1) Carry out seed expansion cultivation of propionibacterium;

[0053] (2) Preparation of liquid species of Propionibacterium;

[0054] (3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;

[0055] (4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;

[0056] (5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;

[0057] (6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.

[0058] The propionibacterium is Propionibacterium acidipropionici DSM25845.

[0059] ...

Embodiment 2

[0070] One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:

[0071] (1) Carry out seed expansion cultivation of propionibacterium;

[0072] (2) Preparation of liquid species of Propionibacterium;

[0073] (3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;

[0074] (4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;

[0075] (5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;

[0076] (6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.

[0077] The propionibacterium is Propionibacterium acidipropionici DSM25845.

[0078] ...

Embodiment 3

[0089] One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:

[0090] (1) Carry out seed expansion cultivation of propionibacterium;

[0091] (2) Preparation of liquid species of Propionibacterium;

[0092] (3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;

[0093] (4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;

[0094] (5) Fermentation ends when the propionate content reaches 22 grams per liter or kilogram;

[0095] (6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.

[0096] The propionibacterium is Propionibacterium acidipropionici DSM25845.

[0097] In the s...

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Abstract

The invention belongs to the technical field of feed mildew preventives and provides a method for producing a propionate mildew preventive through microbial fermentation and a use thereof. Through liquid fermentation, propionibacterium can produce propionate. The method comprises carrying out seed enlarged cultivation on propionibacterium, preparing a liquid propionibacterium strain, inoculating a liquid culture medium with the liquid propionibacterium strain, carrying out fermentation to obtain propionic acid, wherein in fermentation, alkali solution fed-batch is carried out so that the propionic acid and alkali undergo a reaction to produce a propionate, stopping fermentation when the propionate content is 18-25 g / L or g / kg, filtering the fermentation solution through a filter press, taking the filtrate, feeding the filtrate into a spray tower, and carrying out spray drying to obtain powder as a propionate product. The method needs less equipment, has simple processes and can replace the chemical synthesis method.

Description

technical field [0001] The invention belongs to the technical field of antifungal agents for feed, and in particular relates to a production method and application of a propionate antifungal agent produced by microbial fermentation. Background technique [0002] Propionic acid and its salts such as calcium propionate, zinc propionate, and potassium propionate can effectively inhibit mold and aerobic bacillus, and are basically harmless to humans. They are widely used in grains, feed and food. As a food additive, it is a safe and reliable antifungal agent for food and feed approved by the World Health Organization (WHO) and the Food and Agriculture Organization of the United Nations (FAO). It is widely used in the food industry and animal husbandry. Yeast and bacteria have a wide range of antibacterial effects. [0003] At present, the production methods of calcium propionate and ammonium propionate are mostly multi-purpose chemical methods, but this method has obvious disad...

Claims

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Application Information

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IPC IPC(8): C12P7/52A23L3/3508C12R1/01
CPCA23L3/3508C12P7/52
Inventor 张安育
Owner HEFEI HUAGAI BIOTECH CO LTD
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