Single-tube optical field microscope

Abstract

The invention discloses a single-tube optical field microscope used for refocusing imaging and three-dimensional imaging after single exposure of a sample. The single-tube optical field microscope adopts a far field correction objective, and a microlens array and a CMOS (Complementary Metal Oxide Semiconductor) imaging device are inserted into a primary image surface and combined into an optical field sensor to acquire an optical field. Refocusing imaging and three-dimensional imaging are performed on the sample via primary exposure acquisition, optical field recording and imaging calculation. By sufficiently using the characteristic of the microlens array and using the two-dimensional sensor to record four-dimensional optical field information, the depth-of-field problem of the microscope is solved, and mechanical fine focusing is not needed for acquiring a plane image of an object. The single-tube optical field microscope is simple in structure and flexible in application scenes. A target three-dimensional image can be obtained by inverting the acquired four-dimensional optical field information.

Description

technical field [0001] The invention relates to the technical field of optical electron microscopes, in particular to a single-tube light field microscope, which is used for multi-view imaging and three-dimensional imaging of specimens. Background technique [0002] At present, electron microscopes have been widely used in the market, but the higher the magnification of the microscope, the smaller the depth of field. For observing some obvious spatial structures or microorganisms, it is necessary to continuously adjust the focus, and each time the focus can only obtain a local clear image of the specimen. And the market demand for microscopic three-dimensional imaging is also increasing. The invention can not only solve the problem of the depth of field of the microscope, but also invert the three-dimensional image of the specimen through one exposure. Contents of the invention [0003] On the basis of the current development of electron microscopes, the present invention...

AI Technical Summary

Problems solved by technology

[0003] On the basis of the current development of electron microscopes, the present invention proposes a single-tube light field microscope, which effectively solves the contradiction between the depth of field and the magnification of the microscope, records the light field information through one exposure, and inverts multi-view images and three-dimensional images. Compared with the existing Electron microscopy shows obvious advantages

Images