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Fermenting matter by using tremella aurantialba to solidly convert rhizoma dioscoreae and application thereof

A technology for fermentation and solid fermentation, applied in the field of bioengineering, can solve problems such as uncertainty, and achieve the effect of lowering blood sugar, significant effect and quick effect.

Inactive Publication Date: 2017-09-29
张志才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report of using it to produce health care products or medicines containing yam saponin, aureus saponin and polysaccharides, and all of them are on a laboratory scale. Whether the process technology can be used for industrialization is still uncertain

Method used

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  • Fermenting matter by using tremella aurantialba to solidly convert rhizoma dioscoreae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] a. Test tube expansion culture: inoculate the Auricularia slant culture in the potato glucose slant medium, cultivate it at 20°C for 10 days, and then store it at 10°C to obtain the Auricularia slant strain;

[0027] b. Liquid shake flask culture: cut the Auricularia slant strain prepared in step a into a size of 3mm×3mm, pick 3-5 pieces and inoculate them into shake flasks equipped with liquid shake flask culture medium, and culture them at 35°C for 3 days Finally, the liquid shake flask strain was obtained; wherein, every 1L liquid shake flask culture medium was composed of: 60 grams of glucose, 50 grams of corn flour, 20 grams of wheat bran, 25 grams of corn bran, 30 grams of yeast extract, diphosphate Potassium hydrogen 8 grams, magnesium sulfate 8 grams;

[0028]c. Seed tank seed culture: Inoculate the liquid shake flask strains prepared in step b into the seed tank culture medium according to the volume ratio of 20%, at a temperature of 35 ° C, a stirring speed of...

Embodiment 2

[0033] a. Test tube expansion culture: inoculate the Auricularia slant culture in the potato glucose slant medium, cultivate it at 25°C for 6 days, and store it at 5°C to obtain the Auricularia slant strain;

[0034] b. Liquid shake flask culture: cut the Auricularia slant strains prepared in step a into a size of 3mm×3mm, pick 3 pieces and inoculate them into shake flasks equipped with liquid shake flask culture medium, and cultivate them at 20°C for 10 days. Prepare the liquid shake flask strain; Wherein, every 1L liquid shake flask culture medium is composed of: 10 grams of glucose; 10 grams of corn flour; 5 grams of wheat bran; 5 grams of corn bran; 5 grams of yeast extract, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g;

[0035] c. Seed tank seed culture: Inoculate the liquid shake flask strains prepared in step b into the seed tank culture medium according to the volume ratio of 2%, at a temperature of 20°C, a stirring speed of 160r / m, and an air flow of 1.8:...

Embodiment 3

[0040] a. Test tube expansion culture: inoculate the Auricularia slant culture in the potato glucose slant medium, cultivate it at 20-35°C for 3 days, and store it at 1°C to obtain the Auricularia slant strain;

[0041] b. Liquid shake flask culture: Cut the Auricularia slant strains prepared in step a into a size of 3mm×3mm, pick 3-5 pieces and inoculate them into shake flasks equipped with liquid shake flask medium, and culture them at 28°C for 7 days Finally, the liquid shake flask strain was obtained; wherein, every 1L liquid shake flask culture medium was composed of: 30 grams of glucose; 30 grams of corn flour; 12.5 grams of wheat bran, 12.5 grams of corn, 18 grams of yeast extract, dihydrogen phosphate Potassium 5 grams, magnesium sulfate 4 grams;

[0042] c. Seed tank seed culture: Inoculate the liquid shake flask strain prepared in step b into the seed tank culture medium according to the volume ratio of 10%, at a temperature of 28°C, a stirring speed of 110r / m, and a...

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Abstract

The invention relates to a fermenting matter by using tremella aurantialba to solidly convert rhizoma dioscoreae. The fermenting matter is prepared by the following steps of using rice and rhizoma dioscoreae as raw materials; performing liquid flask shaking culture of tremella aurantialba strain, and amplification culture of a liquid seed tank, so as to obtain a liquid strain containing the tremella aurantialba; mixing the liquid strain and the sterilized rice, rhizoma dioscoreae and auxiliary materials, spreading onto a solid fermenting bed, and performing solid fermenting. The fermenting matter has the advantages that the blood glucose can be obviously decreased, and the fermenting matter is a pure natural preparation; compared with the existing hypoglycemic agents, the fermenting matter can quickly take effect, the effect is obvious, and the hypoglycemia is not caused after administration.

Description

technical field [0001] The invention relates to a fermented product of golden ear solid transformation yam, which refers to the use of golden ear fungus species to transform yam saponin, the active ingredient in yam, by solid fermentation technology, and simultaneously degrade carbohydrates such as starch in yam, and synthesize yam saponin, golden yam, etc. Auricular saponins and polysaccharide fermented products belong to the technical field of bioengineering. Background technique [0002] With the development of my country's economy, the improvement of people's living standards and the changes in people's diet structure, especially the increase in high-fat diet intake, the incidence of "three high" diseases such as hypertension, hyperlipidemia and hyperglycemia has increased year by year. The "three high" diseases have become the number one enemy affecting the health of the people, and the number of deaths due to the "three high" diseases ranks first in the death toll of v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L19/10A23L29/00A23L31/00A23L33/105A01G1/04
CPCA23L19/10A23L29/065A23L31/00A23L33/105A23V2002/00A23V2200/328A23V2250/208A23V2250/21
Inventor 张志才
Owner 张志才
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