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Breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin

A technology of wheat root rot and root rot fungus, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of heavy workload, slow progress of disease-resistant breeding, etc., to improve work efficiency and accuracy, The effect of reducing the workload of screening and the application value of large breeding

Inactive Publication Date: 2017-09-29
尹纪岩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for processing hybrids to the crude toxin of root rot pathogen by optimizing the crude toxin of root rot pathogen of wheat root rot disease for the defects of large workload and slow process of disease-resistant breeding for the current wheat root rot resistance identification. Hypersensitive period - the first division stage of fertilized eggs, so as to specifically screen root rot resistance wheat families, greatly improving the work efficiency and accuracy of root rot resistance breeding, and using the crude toxin of root rot bacteria to quickly screen wheat roots Breeding methods for rot-resistant varieties

Method used

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  • Breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin
  • Breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin
  • Breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin

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Effect test

Embodiment 1

[0068] From 2009 to 2010, Zhengmai 9962 (high resistance to wheat root rot in field performance), Xinong 919 (moderate resistance to wheat root rot in field performance) and Zhoumai 18 (high resistance to wheat root rot in field performance) were used in the experimental field of Dongxin Farm, Jiangsu Province. Sensitive wheat root rot) was used to test different anther development stages of wheat treated with crude toxin of root rot fungus. Sowing in the autumn of 2009, and in the first season of 2010, a graded concentration of the root rot fungus crude toxin was set up to treat five different anther development stages of wheat for experiments, namely the unfertilized pollen stage, the first division stage of fertilized eggs, and 5 days after fertilization. Grain embryo development stage, 15 days after fertilization, grain "more than half kernel" stage, 25 days after fertilization, grain filling stage. Due to the different sensitivity to crude toxins of root rot pathogens in ...

Embodiment 2

[0084] From 2010 to 2011, a total of 12 materials with high resistance to wheat root rot reported by solicited research and high-resistance materials screened by our unit for many years in field investigations were selected. The crude toxin treatment test of root rot pathogen was carried out in the experimental field of Dongxin Farm, Jiangsu Province. The test period was selected as the first division period of wheat fertilized eggs. In view of the fact that the crude toxin stock solution concentration of 10% dilution ratio has a significant difference in root rot resistance among wheat varieties in the treatment experiment of root rot pathogen crude toxin in different anther development stages of wheat from 2009 to 2010, so the concentration of 10% dilution ratio is preferred The crude toxin was tested.

[0085] At the same time, the field disease nursery resistance identification test was carried out. The soil was loamy soil with medium fertility. Before plowing, 40 kg of wh...

Embodiment 3

[0105] From 2011 to 2016, using the technical route of the present invention, Wangshuibai was selected as the hybrid male parent, and the root rot resistance of the breeding main parent W0962 was successfully improved, and 3 new root rot resistant wheat lines were bred.

[0106] (1) Select W0962 and Wangshuibai as hybrid female parent and male parent respectively:

[0107] W0962 is the main parent of wheat breeding used by our unit for many years. It has many advantages such as high yield, excellent rice quality, and early maturity. The rate is higher, and the number of ears per mu is higher; the greening and jointing is fast in spring, the appearance is handsome, the flag leaves are wide and raised, the plant type is moderate, the ears are large, cold resistance, yellow fall, and the grains are semi-horny, fuller, and the grain weight is higher. high. The stems are tough, and light lodging has little effect on the filling; the three elements of yield are coordinated, and the...

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Abstract

The invention discloses a breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin. The method comprises the following steps of (1) selecting to-be-improved wheat variety / strain and wheat root rot resistance source and hybridizing to obtain F1; (2) backcrossing the F1 to obtain BCF1; (3) sowing male and female parents and the BCF1 at different stages; (4) treating the male and female parents by using root rot fungus crude toxin of gradient concentrations and selecting resistance screening pressure of the root rot; (5) treating the BCF1 by using the resistance screening pressure of the root rot to obtain BCF2; and (6) taking BCF2 for anther culture to obtain an anther culture family and screening according to root rot resistance and agronomic characters to obtain a new wheat strain with root rot resistance. Hybrid species are treated by selecting the root rot fungus crude toxin with a proper concentration at the hypersensitive stage of the root rot fungus crude toxin and the first division stage of zygote, thereby specifically screening the resistance wheat family for the root rot and greatly improving the work efficiency and the accuracy of resistant breeding of the root rot.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method of treating the hypersensitive stage of the hybrid species to the crude toxin of the root rot fungus by optimizing the crude toxin of the root rot fungus at a suitable concentration-the first division stage of the fertilized egg, thereby specifically screening the roots. A wheat family with resistance to rot, and a breeding method for rapidly screening root rot resistant varieties of wheat by using the crude toxin of root rot fungus, which greatly improves the work efficiency and accuracy of root rot resistance breeding. Background technique [0002] Wheat root rot, also known as root rot leaf spot or black germ disease, is a soil-borne fungal disease that can occur during the whole growth period of wheat, can damage the rhizome of wheat, cause root rot, and can also harm wheat The leaves of wheat can cause leaf spots, and can also harm wheat grains and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00A01G7/06
CPCA01H1/02A01G7/06A01H1/04A01H4/008
Inventor 尹纪岩
Owner 尹纪岩
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