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Method for inducing proliferation and erythroid differentiation of hematopoietic stem progenitor cells and its application

A technology of hematopoietic stem cells and erythroid differentiation, applied in biochemical equipment and methods, blood/immune system cells, animal cells, etc., can solve the problem of low efficiency of proliferation-induced erythroid differentiation, and achieve the promotion of induction and erythroid differentiation, Promoting proliferation and low price effects

Active Publication Date: 2020-10-23
SOUTH CHINA INSTITUDE OF BIOMEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the current expansion and induction system of erythroid progenitor cells still has problems such as low efficiency of proliferation and induction of erythroid differentiation, which still needs to be improved

Method used

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  • Method for inducing proliferation and erythroid differentiation of hematopoietic stem progenitor cells and its application
  • Method for inducing proliferation and erythroid differentiation of hematopoietic stem progenitor cells and its application
  • Method for inducing proliferation and erythroid differentiation of hematopoietic stem progenitor cells and its application

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Embodiment 1

[0080] 1. Materials

[0081] Cord blood mononuclear cells: mix hydroxyethyl starch with cord blood (the cord blood is the cord blood of a healthy fetus with full-term pregnancy, and comes from the Armed Police General Hospital.) at a ratio of 1:3-1:5, and settle red blood cells for 20 -30min, take the supernatant, centrifuge, suspend the cells in normal saline, slowly add an equal volume of cell suspension to the test tube added with human lymphocyte separation medium, centrifuge, and separate the middle buffy coat, that is, mononuclear cells.

[0082] Stemspan expansion medium: Stemspan medium containing 50ng / ml SCF, 10ng / ml TPO, 20ng / ml IL-3, 10ng / ml IL-6, 50ng / ml Flt3.

[0083] SCGM expansion medium: SCGM medium containing 50ng / ml SCF, 10ng / ml TPO, 20ng / ml IL-3, 10ng / ml IL-6, 50ng / ml Flt3.

[0084] Stemspan induction medium: Stemspan medium containing 100ng / ml SCF, 40ng / μl IGF-1, 5U / ml EPO, 100μg / ml transferrin, 1μM dexamethasone, 2mM glutamine and lipid.

[0085] SCGM in...

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Abstract

The invention discloses a method for inducing proliferation and erythroid differentiation of hematopoietic stem and progenitor cells and application of the method. The method for inducing the proliferation and the erythroid differentiation of the hematopoietic stem and progenitor cells includes carrying out first culture on hematopoietic stem cells by the aid of first culture media to obtain proliferated hematopoietic stem cells; carrying out second culture on the proliferated hematopoietic stem cells by the aid of second culture media to obtain erythroid progenitor cells. The first culture media are Stemspan serum-free culture media or SCGM (stem cell growth medium) serum-free culture media with first additives, and the first additives are selected from at least one type of recombinant human stem cell factors, recombinant human erythropoietin, interleukin-3, interleukin-6 and FMS fins-like tyrosine kinase. The second culture media are SCGM serum-free culture media with second additives, and the second additives are selected from at least one type of recombinant human stem cell factors, insulin-like growth factors 1, recombinant human erythropoietin, transferrin, dexamethasone, glutamine and lipoid.

Description

technical field [0001] The present invention relates to the field of bioengineering, in particular, a method for inducing the proliferation and erythroid differentiation of hematopoietic stem progenitor cells and its application, more specifically, a method for inducing the proliferation and erythroid differentiation of hematopoietic stem progenitor cells, and a method for inducing hematopoietic stem progenitor cells Kits for cell proliferation and erythroid differentiation and systems for inducing proliferation and erythroid differentiation of hematopoietic stem progenitor cells. Background technique [0002] In recent years, due to the shortage of blood sources and the occurrence of blood transfusion-related diseases, people expect to find safer and sufficient blood sources. Studies have shown that stem cells can induce erythroid differentiation into red blood cells in vitro and can be used as a new blood source. In addition to mature erythrocytes, erythroid progenitor ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0789C12N5/078
CPCC12N5/0641C12N5/0647C12N2500/24C12N2500/32C12N2500/36C12N2500/90C12N2501/105C12N2501/125C12N2501/14C12N2501/2303C12N2501/2306C12N2501/727C12N2506/11
Inventor 裴雪涛陈琳谢小燕房芳岳文李艳华吕洋黄安平张博文刘大庆何丽娟
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
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