Rapeseed sourced ZCP (zinc chelating peptide) as well as preparation method and application thereof

Abstract

The invention discloses rapeseed sourced ZCP (zinc chelating peptide). The amino acid sequence of the ZCP is Ala-Ser-His. The invention further discloses a preparation method of the rapeseed sourced ZCP. The preparation method comprises the following steps: preparation of rapeseed protein, two-step hydrolysis of the rapeseed protein, affinity chromatography, gel filtration chromatography, reversed-phase high-performance liquid chromatographic separation and purification. The invention further discloses an application of the rapeseed sourced ZCP to preparation of an intermediate of a zinc supplement preparation. The rapeseed sourced ZCP has high zinc chelating activity and can serve as a good intermediate of a fourth-generation zinc supplement preparation, and an ideal carrier is provided for further development of fourth-generation zinc supplement products with high absorptivity and high biological availability.

Description

technical field [0001] The invention belongs to the technical field of deep processing of rapeseed and its by-products, and in particular relates to a rapeseed-derived zinc chelating peptide and a preparation method thereof, as well as the application of the zinc chelating peptide in preparing zinc supplement products. Background technique [0002] Zinc (Zn) is one of the essential trace elements for the human body. Studies have shown that zinc is an essential component of nearly 2,000 transcription factors in the body, related to the activity of more than 300 metabolic enzymes, and affects the body's immune system and redox balance. , can be used as an antioxidant factor and an anti-inflammatory factor in pathological conditions such as chronic vascular disease, cancer, neurodegeneration, immune disorder and aging. [0003] Zinc chelating peptide (zinc chelating peptide, ZCP) is a kind of trace metal ion chelating peptide, it is the ligand of zinc ion in the fourth generati...

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Problems solved by technology

[0004] The sources of existing zinc chelating peptides mainly include wheat germ (such as patent CN 103626847 B "a kind of wheat germ protein source zinc chelating peptide and its preparation method"), casein (such as "casein phosphopeptide zinc chelating peptide Separation and Characterization of Structural Properties", China National Knowledge Network, website http: / / www.cnki.net / kcms / detail / 11.1802.TS.20170517.1315.038.html), hazelnut (such as "Preparation, separation and purification of hazelnut chelating peptide and nature research", China National Knowledge Network, master's and doctoral dissertation, website http: / / cdmd.cnki.com.cn / Article / CDMD-10157-1016143419.htm), horse-faced puffer fish skin (such as CN 104928337 A "horse-faced Puffer fish skin zinc chelating peptide"), shrimp offal (such as patent CN 105192718A "shrimp offal zinc chelating peptide use), monocyclic spiny (such as patent CN106086129A "a kind of monocyclic spiny viscera protein source zinc chelation Peptide preparation method"), etc., wherein, the wheat germ protein source zinc chelating peptide is 10 peptides and 12 peptides, the casein phosphopeptide zinc chelating peptide is a polypeptide mixture, the shrimp leftover zinc chelating peptide is 8 peptides, horse noodles The zinc chelating peptide of puffer fish skin is 10 peptides, and the zinc chelating peptide derived from the visceral protein of monocyclic echinacea is 5 peptides; and the peptide with amino acid residues greater than 6 is difficult to be absorbed by the human body, unless it is specific, when it is made into a supplement After taking zinc food or health care products, the human body's absorption rate and biological utilization of zinc ions are quite limited.

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