A kind of rapeseed source zinc chelating peptide and its preparation method and application

Abstract

The invention discloses a rapeseed source zinc chelating peptide, the amino acid sequence of the zinc chelating peptide is: Ala-Ser-His; the invention also discloses a preparation method of the rapeseed source zinc chelating peptide, Including: preparation of rapeseed protein, two-step hydrolysis of rapeseed protein, affinity chromatography, gel filtration chromatography, reversed-phase high-performance liquid chromatography separation and purification; the invention also discloses that the rapeseed-derived zinc chelating peptide is Application in the preparation of zinc supplement intermediates. The rapeseed zinc chelating peptide prepared by the present invention has high zinc chelating activity and can be used as a good intermediate of the fourth-generation zinc supplement, and is used for the further development of the fourth-generation zinc supplement with high absorption rate and high biological utilization rate. The product provides an ideal carrier.

Description

technical field [0001] The invention belongs to the technical field of deep processing of rapeseed and its by-products, and in particular relates to a rapeseed-derived zinc chelating peptide and a preparation method thereof, as well as the application of the zinc chelating peptide in preparing zinc supplement products. Background technique [0002] Zinc (Zn) is one of the essential trace elements for the human body. Studies have shown that zinc is an essential component of nearly 2,000 transcription factors in the body, related to the activity of more than 300 metabolic enzymes, and affects the body's immune system and redox balance. , can be used as an antioxidant factor and an anti-inflammatory factor in pathological conditions such as chronic vascular disease, cancer, neurodegeneration, immune disorder and aging. [0003] Zinc chelating peptide (zinc chelating peptide, ZCP) is a kind of trace metal ion chelating peptide, it is the ligand of zinc ion in the fourth generati...

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Problems solved by technology

[0004] The sources of existing zinc chelating peptides mainly include wheat germ (such as patent CN 103626847 B "a kind of wheat germ protein source zinc chelating peptide and its preparation method"), casein (such as "casein phosphopeptide zinc chelating peptide Separation and Characterization of Structural Properties", China National Knowledge Network, website http: / / www.cnki.net / kcms / detail / 11.1802.TS.20170517.1315.038.html), hazelnut (such as "Preparation, separation and purification of hazelnut chelating peptide and nature research", China National Knowledge Network, master and doctoral dissertation, website http: / / cdmd.cnki.com.cn / Article / CDMD-10157-1016143419.htm), horse-faced puffer fish skin (such as CN104928337 A "horse-faced puffer Fish skin zinc chelating peptide"), shrimp offal (such as patent CN 105192718A "shrimp offal zinc chelating peptide use), monocyclic spiny cartilage (such as patent CN106086129A "a kind of monocyclic spiny cartilage visceral protein source zinc chelating peptide The preparation method of ") etc., wherein, the wheat germ protein source zinc chelating peptide is 10 peptides and 12 peptides, the casein phosphopeptide zinc chelating peptide is a polypeptide mixture, the shrimp offal zinc chelating peptide is 8 peptides, and horse-faced fish The fish skin zinc chelating peptide is a 10-peptide, and the zinc chelating peptide derived from the visceral protein of the monocyclic thorn is a 5-peptide; while the amino acid residue is greater than 6 peptides, it is difficult to be absorbed by the human body, unless it is specific, when it is made into a zinc supplement After taking food or health care products, the human body's absorption rate and biological utilization of zinc ions are quite limited

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