A kind of reactive mercapto compound probe and preparation method thereof

Abstract

The invention discloses a reactive sulfhydryl compound probe and a preparation method of the probe. A sensing molecule releases a rhodamine dye molecule monomer based on a thiol participated disulfide bond cleavage reaction, the fluorescence intensity is significantly improved, an obvious color change is accompanied, and selected interfering ions and the like almost have no influence on a detection effect, so that specific recognition response to a sulfhydryl compound is realized, and a detection limit reaches 0.124 micrometers.

Description

Technical field [0001] The invention belongs to the technical field of organic synthesis, and specifically relates to a reactive sulfhydryl compound probe and a preparation method thereof. Background technique [0002] Many proteins and small molecules in organisms contain sulfhydryl groups, such as glutathione (GSH), cysteine ​​(Cys) and homocysteine ​​(Hcy). The sulfhydryl group plays an extremely important role in the antioxidant system of the cells in the organism. It can prevent the cells in the organism from oxidative damage. Medical research has shown that glutathione and cysteine ​​are related to many diseases, such as renal failure, Alzheimer's disease, etc. The changes in their content in the body can be used as the basis for the diagnosis of these diseases, so the development of selective detection of cysteine The detection method of acid is particularly important. [0003] At present, there are many methods for determining the content of amino acids, such as colorimet...

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Problems solved by technology

The colorimetric method is based on the color reaction to generate colored compounds, and generally includes two steps: first, select an appropriate color reagent to react with the component to be tested to form a colored compound, and then compare or measure the color of the colored compound Depth, in this process, the acidity of the solution, the amount of chromogen, temperature, solvent, etc. all have an impact on the color reaction, and the sensitivity and accuracy of the measurement are poor; It is a widely used method, but it is sensitive to the acidity and temperature of the sample solution and is not easy to operate; the enzyme cycle method is a method for amplifying the measured substance by using the substrate specificity of the enzyme, but the amount of tool enzyme used is large and the cost is relatively high. High; capillary electrophoresis is an electrophoretic separation analysis method that uses elastic quartz capillary as the separation channel, uses high-voltage direct current electric field as the driving force, and realizes separation according to the differences in mobility and distribution behavior among the components in the sample. The diameter of the capillary is small , so that the optical path is too short, the sensitivity is low, and the electroosmosis will change due to the composition of the sample, which will affect the reproducibility of the separation; the HPLC method has high accuracy and simple sample processing, but it is limited by the chromatographic column and takes a long time

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