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Bacillus for promoting enzymolysis of eichhornia crassipes substrate to produce sugar and application thereof

A technology of bacillus and water hyacinth, which is applied in the field of solid waste resource utilization, can solve problems such as less reports, and achieve the effects of improving sugar production, enriching genetic resources, and economical effects.

Active Publication Date: 2017-09-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently reported degumming strains include Actinomyces, Cellulomonas, Shigella and Bacillus subtilis, etc. These are all degumming strains for hemp, but there are fewer reports on Bacillus axarquiensis

Method used

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  • Bacillus for promoting enzymolysis of eichhornia crassipes substrate to produce sugar and application thereof
  • Bacillus for promoting enzymolysis of eichhornia crassipes substrate to produce sugar and application thereof
  • Bacillus for promoting enzymolysis of eichhornia crassipes substrate to produce sugar and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The present embodiment has carried out the preliminary screening of degumming microorganism, and concrete screening process is as follows:

[0033]Pond water, pond bottom mud, South Lake water, South Lake bottom mud, Yezhi Lake water, Yezhi Lake bottom mud, fresh pig manure and vigorously growing water hyacinth hyacinth strains were collected from the ponds growing water hyacinth near the pig farm of Huazhong Agricultural University. Wash the water hyacinth hyacinth strain, cut it into small pieces of 1-2 cm, and air-dry the surface moisture. Weigh 10g of bottom mud (containing water) in a sterile Erlenmeyer flask equipped with 90mL of sterilized water, shake at room temperature for 1 hour at an oscillation speed of 125r / min, and then filter with two layers of gauze to obtain a bottom mud suspension;

[0034] Weigh 5g of air-dried fresh water hyacinth in a sterile Erlenmeyer flask, add 30mL of water sample and sediment suspension respectively, then add 270mL of sterile ...

Embodiment 2

[0036] In this embodiment, the degummed microorganisms obtained in Example 1 have been re-screened, and the specific process is as follows:

[0037] The strains obtained from the primary screening were inoculated into the degumming medium respectively, and cultivated for 3 days at 35°C and 145rpm, and the liquid phosphate-dissolving medium without adding any bacteria was used as the control group. After the cultivation is completed, the solid-liquid separation, the total reducing sugar content of the liquid is measured by the DNS method, the solid is dried at 65 °C, and the enzymatic hydrolysis is carried out with a cellulase load of 30 FPU / g substrate for 72 hours, and the reducing sugar production in the enzymatic solution is detected by the DNS method . The result is as figure 1 As shown, the above-mentioned 8 strains screened by the present invention all have promoting effect on the enzymatic hydrolysis sugar production of substrate after the above-mentioned 8 strains scr...

Embodiment 3

[0039] This embodiment makes identification to strain CTS-BQ1

[0040] (1) Colony morphology and properties of strain CTS-BQ1

[0041] The colony is dirty white, the surface is smooth and moist, and the edges are irregular, and it is Gram-positive bacteria.

[0042] (2) Molecular biological identification of strain CTS-BQ1

[0043] The CTS-BQ1 strain obtained from the above screening was identified by molecular biology methods, and the DNA of the strain was amplified by PCR using universal primers 27F and 1492R. The amplified product was sequenced by a sequencing company, and the sequence was compared with the GenBank database. BLAST comparison of the sequences in the strain showed that the similarity between the strain and Bacillus axarquiensis was more than 99%. Combined with morphological characteristics, cultural characteristics and 16S rRNA sequence analysis, the strain was identified as Bacillus axarquiensis.

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Abstract

The invention provides a bacillus (Bacillus axariensis) CTS-BQ1 for promoting enzymolysis of an eichhornia crassipes substrate to produce sugar, and further provides a method for promoting the enzymolysis of the eichhornia crassipes substrate to produce the sugar and a microbial agent for promoting the enzymolysis of the eichhornia crassipes substrate to produce the sugar. The strain provided by the invention enriches genetic resources of wild degumming bacteria and enlarges a back-up library of the whole genome breeding of the degumming bacteria. The bacillus is more effective in improving sugar yield of the enzymolysis of the eichhornia crassipes than white rot fungi with a reported effect, is more efficient and economical than commercial pectinase, has a remarkable effect, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of resource utilization of solid waste, and in particular relates to a strain of Bacillus axarquiensis CTS-BQ1 and its pretreatment function for promoting enzymatic hydrolysis of water hyacinth to produce sugar, which can be applied to the high-value utilization of water hyacinth pre-processing. Background technique [0002] As a invasive aquatic plant, water hyacinth, on the one hand, grows fast and has strong adaptability, and is easy to pollute water bodies after corruption; With high cellulose content, it is a relatively potential raw material for the production of bioenergy substances. However, due to its high moisture content, lignin wraps cellulose, which makes it difficult to release and is not easy to be used. Its pretreatment cost is high, and the efficiency of sugar and ethanol production is low. Biological pretreatment has the advantages of mild action conditions, low energy consumption, less environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/00C12R1/07
CPCC12N1/20C12P19/00C12N1/205C12R2001/07
Inventor 周文兵杨庆肖乃东朱端卫肖凯张超奇冯伟
Owner HUAZHONG AGRI UNIV
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