Rat pulmonary cell fibrosis model

A fibrosis and cell technology, applied in the medical field, can solve the problems of large exogenous trauma, large data impact, time-consuming and other problems, and achieve the effects of low mortality, convenient operation and high success rate.

Inactive Publication Date: 2017-09-01
WENZHOU PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many existing methods for constructing animal models of pulmonary fibrosis, mainly including: tracheotomy, tracheal intubation, forehead mirror-guided perfusion, intraperitoneal injection, nasal administration, intravenous infusion of chemical drugs, and oropharyngeal administration There are many modeling methods, but each has its own shortcomings. For example, tracheotomy has a large impact on the exogenous trauma of the specimen mouse, and the operation is complicated, which has a great impact on the relevant laboratory data, and the operation is time-consuming.

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Embodiment 1

[0016] A rat lung cell fibrosis model, the establishment method is as follows: the rat is weighed, anesthetized by intraperitoneal injection of anesthesia, placed in a cage, and allowed to faint naturally, and for the administration group, a sample gun is used to pass through the nasal cavity The way of administration is given to the medicine, which contains amiodarone and naphazoline hydrochloride with a concentration of 3mg / ml and 0.03mg / ml respectively, and the dosage of the amiodarone is 3mg / kg body weight, Remove the hair from the neck of the rat, prepare a transparent plastic bag with an elastic fastening belt on the edge of the mouth, cover the transparent plastic bag on the head of the rat, and make the elastic fastening belt on the edge of the mouth of the transparent plastic bag Sleeve it at the neck of the rat, set two holes on the transparent plastic bag, insert an air guide tube into one of the holes, the other end of the air guide tube is connected with a three-wa...

Embodiment 2

[0018] A rat lung cell fibrosis model, the establishment method is as follows: the rat is weighed, anesthetized by intraperitoneal injection of anesthesia, placed in a cage, and allowed to faint naturally, and for the administration group, a sample gun is used to pass through the nasal cavity The way of administration is given to the medicine, which contains amiodarone and naphazoline hydrochloride with a concentration of 4mg / ml and 0.04mg / ml respectively, and the dosage of the amiodarone is 4mg / kg body weight, Remove the hair from the neck of the rat, prepare a transparent plastic bag with an elastic fastening belt on the edge of the mouth, cover the transparent plastic bag on the head of the rat, and make the elastic fastening belt on the edge of the mouth of the transparent plastic bag Sleeve it at the neck of the rat, set two holes on the transparent plastic bag, insert an airway tube into one of the holes, the other end of the airway tube is connected with a three-way valv...

Embodiment 3

[0020]A rat lung cell fibrosis model, the establishment method is as follows: the rat is weighed, anesthetized by intraperitoneal injection of anesthesia, placed in a cage, and allowed to faint naturally, and for the administration group, a sample gun is used to pass through the nasal cavity The way of administration is given to the medicine, which contains amiodarone and naphazoline hydrochloride with a concentration of 5mg / ml and 0.06mg / ml respectively, and the dosage of the amiodarone is 5mg / kg body weight, Remove the hair from the neck of the rat, prepare a transparent plastic bag with an elastic fastening belt on the edge of the mouth, cover the transparent plastic bag on the head of the rat, and make the elastic fastening belt on the edge of the mouth of the transparent plastic bag Sleeve it at the neck of the rat, set two holes on the transparent plastic bag, insert an air guide tube into one of the holes, the other end of the air guide tube is connected with a three-way...

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Abstract

The invention discloses a rat pulmonary cell fibrosis model. For a medication administration group, a medicine is administrated in an intranasal administration mode with an administration gun, the medicine comprises amiodarone and naphazoline hydrochloride, the amiodarone is adopted as an inducing agent for establishing the rat pulmonary cell fibrosis model, and the content of hdroxyproline is tested through high-resolution CT (Computed Tomography) observation. According to the rat pulmonary cell fibrosis model disclosed by the invention, the amiodarone is injected in a noninvasive intranasal administration mode to establish a lung fibrosis animal model, the operation is convenient, the success rate is high, small damage can be made to rats in the administration process, exogenous damage caused by tracheotomy of the rats is reduced, the death rate of the rats in the administration process is small, and medicines can be fed into tracheas completely.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a rat lung cell fibrosis model. Background technique [0002] Amiodarone, also known as amiodarone, is an iodine-containing phenylpropanfuran derivative that is widely used in clinical practice as a class III antiarrhythmic drug, mainly for the treatment of ventricular arrhythmias and atrial fibrillation. Its side effects are mainly pulmonary toxicity, thyroid toxicity, cardiotoxicity, digestive system toxicity, etc. Among them, pulmonary toxicity is the most serious, with an incidence rate of 1%-10%. Pulmonary toxicity is mainly manifested as pulmonary fibrosis. Measures, its mortality rate is as high as 33%, seriously affecting people's life and health. However, amiodarone has a definite and stable antiarrhythmic effect, and there is no other similar drug to replace it, and it is still widely used clinically. Therefore, elucidating the mechanism of amiodarone-induced pulmonary fibrosi...

Claims

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Application Information

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IPC IPC(8): A61K31/4174A61K31/343A61K31/661A61K31/4245A61P11/00
CPCA61K31/4174A61K31/343A61K31/4245A61K31/661A61K2300/00
Inventor 陈皓王毅姜文兵陈晓曙吴悠扬林伟朱宁胡逸人李林锦
Owner WENZHOU PEOPLES HOSPITAL
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