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A surface-enhanced Raman detection method for bisphenol A residues in milk

A surface-enhanced Raman and detection method technology, applied in Raman scattering, preparation of test samples, material excitation analysis, etc., can solve the problems of complex pretreatment and high cost, and achieve improved sensitivity, less sample volume, and rapid detection Effect

Inactive Publication Date: 2019-09-06
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention aims to solve the technical problem of complicated pretreatment and high cost in the existing method for detecting bisphenol A residue in milk, and provides a surface-enhanced Raman detection method for bisphenol A residue in milk

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  • A surface-enhanced Raman detection method for bisphenol A residues in milk
  • A surface-enhanced Raman detection method for bisphenol A residues in milk
  • A surface-enhanced Raman detection method for bisphenol A residues in milk

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specific Embodiment approach 1

[0021] Embodiment 1: The surface-enhanced Raman detection method of bisphenol A residue in milk according to the present embodiment is carried out according to the following steps:

[0022] 1. Prepare a gold nanoparticle (Au NPs) sol, and then modify the Au NPs with a halide aqueous solution to obtain a halide-modified Au NPs-modified substrate with SERS activity;

[0023] 2. Add methanol / water mixed solvent with a volume ratio of 3:1 to the milk sample to be tested, ultrasonically treat for 1-3 minutes, then centrifuge for 8-15 minutes, remove the supernatant and evaporate to dryness naturally at room temperature to obtain the residue ; Add methanol / water mixed solvent with a volume ratio of 3:1 to the residue and ultrasonically treat it for 1 to 3 minutes, then centrifuge for 5 to 8 minutes, remove the supernatant, and use 0.45 μm and 0.22 μm aqueous membranes in sequence The supernatant is filtered to obtain the supernatant of the milk sample to be tested;

[0024]3. The h...

specific Embodiment approach 2

[0026] Embodiment 2: This embodiment differs from Embodiment 1 in that in step 1, a gold nanoparticle (Au NPs) sol is prepared, and then the Au NPs are modified with an aqueous halide solution to prepare halide-modified Au with SERS activity. The specific steps of NPs modified substrate are as follows:

[0027] a. Add 1 mL of 1% HAuCl 4 Add it into a three-necked flask with 99mL of deionized water, and heat it under magnetic stirring to dissolve it; when the solution is heated to a boiling state of 94°C, add 4mL of a 1% sodium citrate aqueous solution in the three-necked flask. The solution gradually changed from colorless and transparent to lavender and purple, and finally a wine-red transparent sol was formed; the transparent sol was kept at a slight boiling state at 96°C for 15 minutes, then stopped heating, and cooled naturally to room temperature to obtain gold sol;

[0028] b. Centrifuge the Au NPs sol to obtain the Au NPs concentrate; fully mix the halide aqueous solut...

specific Embodiment approach 3

[0029] Specific embodiment 3: The difference between this embodiment and specific embodiment 2 is: in step b, the centrifugation time is 5-10 min, and the centrifugation speed is 5000-10000 rpm; the volume ratio of Au NPs concentrate and halide aqueous solution is mixed 1:1. Others are the same as in the second embodiment.

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Abstract

The invention discloses a surface enhanced Raman detection method for detecting residues of bisphenol A in milk, and relates to a method for detecting residues of bisphenol A in milk, which aims at solving the technical problems of complicated pretreatment and high cost in the existing method for detecting the residues of bisphenol A in milk. The method comprises the following steps of preparing a gold nanoparticle sol, and then modifying by using a halogen water solution, so as to prepare a modified substrate with SERS (surface enhanced Raman scattering) activity; treating a to-be-detected milk sample by a methanol and water mixed solvent, so as to obtain supernatant of the to-be-detected milk sample; mixing the modified substrate with SERS activity and the supernatant of the to-be-detected milk sample, adding a sulfate water solution as a gathering agent, and loading into a glass dot sample capillary tube, so as to obtain a to-be-detected milk sample detection tube; testing the surface enhanced Raman spectrum, and comparing with a Raman spectrum of a biphonel A standard product, if a feature signal peak of the biphonel A standard product occurs, the biphenol A exists in the to-be-detected milk sample. The surface enhanced Raman detection method has the advantages that the detection limit reaches 4.3*10<-9>mol / L, and the method can be applied to the field of biological detection.

Description

technical field [0001] The invention relates to a detection method for bisphenol A residue in milk. Background technique [0002] Bisphenol A (BPA) is an important organic chemical raw material, which is widely used in the production of plastic products such as epoxy resin and polycarbonate. In plastic products, adding bisphenol A can make it colorless, transparent, durable, anti-oxidation, lightweight and impact-resistant, so it is widely used in the manufacture of plastic (milk) bottles, food and beverages (especially dairy products) The inner side of the package is coated. The use of BPA in food packaging materials will inevitably lead to the residue of BPA in food. Animal experiments have shown that bisphenol A is one of the main endocrine disruptors, which can imitate estrogen-like effects, disrupt the endocrine function of normal estrogen, cause precocious puberty and metabolic disorders, induce breast tumors, and reduce immune function. Damage the reproductive syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 杨立滨江欣杨铭李秀玲沈宇赵志宇佟德成陈永亮
Owner JIAMUSI UNIVERSITY
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