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Surface enhanced raman detection method on penicillin drug in milk

A technology of surface-enhanced Raman and medium penicillin, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of increased experimental costs and achieve the effects of rapid detection, improved sensitivity, and easy-to-obtain sample volume

Inactive Publication Date: 2016-12-07
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This detection method uses a purification kit for pretreatment, and uses horseradish peroxidase-labeled goat anti-rabbit polyclonal antibody (secondary antibody) to construct a biosensor, which greatly increases the experimental cost.
[0007] So far, the detection of penicillin drug molecules in milk based on surface-enhanced Raman scattering has not been reported

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  • Surface enhanced raman detection method on penicillin drug in milk
  • Surface enhanced raman detection method on penicillin drug in milk
  • Surface enhanced raman detection method on penicillin drug in milk

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specific Embodiment approach 1

[0021] Embodiment 1: The surface-enhanced Raman detection method of penicillin drug in milk according to the present embodiment is carried out according to the following steps:

[0022] 1. Prepare a silver nanoparticle (Ag NPs) sol with SERS activity, and then use layer-by-layer self-assembly technology to prepare an Ag NPs assembly substrate on a glass substrate;

[0023] 2. Add organic matter / water mixed solvent to the milk sample to be tested, sonicate for 2-5 minutes, then centrifuge for 10-20 minutes, remove the supernatant and evaporate to dryness naturally at room temperature to obtain a residue; then add Deionized water and ultrasonic treatment for 2 to 5 minutes, then centrifuged for 5 to 10 minutes, removed the supernatant, and successively filtered the supernatant with 0.45 μm and 0.22 μm aqueous membranes to obtain the supernatant of the milk sample to be tested;

[0024] 3. Immerse the glass substrate assembled with Ag NPs in the supernatant of the milk sample to ...

specific Embodiment approach 2

[0026] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, a silver nanoparticle (Ag NPs) sol with SERS activity is prepared, and then the Ag NPs assembly substrate is prepared on a glass substrate by layer-by-layer self-assembly technology The specific steps are as follows:

[0027] a. AgNO 3 Add it into a three-necked flask with deionized water, heat it under magnetic stirring to dissolve it; continue heating, when the solution is heated to a boiling state of 98-100°C, add a 1% sodium citrate aqueous solution, stir to form a transparent Sol; the transparent sol is kept in a slightly boiling state at a temperature of 93-97°C for 50-60min, and then the heating is stopped, and the silver sol is naturally cooled to room temperature to obtain a silver sol; wherein AgNO 3 The ratio of the mass of the sodium citrate to the volume of a 1% sodium citrate aqueous solution is 9mg: 1mL;

[0028] b. Boil the clean glass substrate i...

specific Embodiment approach 3

[0029] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the organic / water mixed solvent in step two is a mixed solution of absolute ethanol, chloroform and water; or a mixed solution of absolute ethanol, acetone and water ; Others are the same as the specific embodiment one or two.

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Abstract

The invention discloses a surface enhanced raman detection method on penicillin drug in milk, relating to a detection method of penicillin drug in milk. The invention provides a surface enhanced raman detection method on penicillin drug in milk. The method comprises the following steps: I. preparing silver nanoparticle sol with SERS activity, and then preparing Ag NPs assembled substrate on a glass substrate by utilizing self-assembling technology layer by layer; II. treating to-be-detected milk sample through organic matter / water mixed solution, to obtain to-be-detected milk sample supernatant; III. placing the glass substrate assembled with Ag and NPs into the to-be-detected milk sample supernatant for soaking, taking out and airing, to obtain a to-be-detected milk sample detection slide; and IV. testing the surface enhanced ramn spectrum, obtained from the step III, through a ramn spectrograph, and comparing the spectrum with the ramn spectrum of penicillin drug standard, so that the qualitative detection on the penicillin drug in milk can be realized, and the qualitative detection limit is 10<-7>mol / L.

Description

technical field [0001] The invention relates to a method for detecting penicillin in milk. Background technique [0002] Penicillin belongs to β-lactam antibiotics, and it is one of the most widely used and highly effective antibacterial drugs to treat various bacterial infectious diseases. It is widely used in human disease treatment and animal husbandry. However, the abuse of antibiotics is widespread, and the abuse of penicillin can cause severe allergic reactions and bacterial drug resistance, and even cause human death. In terms of animal husbandry, penicillin drugs are often used to prevent and treat cow mastitis and other diseases, so there will be penicillin residues in milk, which poses a potential hazard to human health. In order to ensure food safety, it is necessary to carry out detection research on trace penicillin drug molecule residues in food, especially the detection of penicillin drug molecule in milk. Traditionally, the analysis methods of penicillin dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨立滨江欣杜娟李秀玲张云杰陈永亮杨铭张秀梅
Owner JIAMUSI UNIVERSITY
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