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Preparation method and application of electrochemical biosensor based on coenzyme A-Au(I) coordination polymer

A technology of coordination polymers and biosensors, applied in the field of detection applications, can solve unseen and undisclosed problems, achieve the effects of stable sensors, accelerated electron transfer, and improved detection sensitivity

Active Publication Date: 2017-08-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are no reports about the simulated peroxidase properties of CoA-Au(I) coordination polymers at home and abroad, and the research on electrochemical sensors based on graphene and CoA-Au(I) coordination polymer modified electrodes And there are no relevant reports on the detection of hydrogen peroxide and extremely small amounts of hydrogen peroxide in biological samples by electrochemical methods.

Method used

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  • Preparation method and application of electrochemical biosensor based on coenzyme A-Au(I) coordination polymer
  • Preparation method and application of electrochemical biosensor based on coenzyme A-Au(I) coordination polymer
  • Preparation method and application of electrochemical biosensor based on coenzyme A-Au(I) coordination polymer

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Embodiment 1

[0035] A method for preparing an electrochemical biosensor based on the analog peroxidase activity-like nucleic acid CoA-Au(I) coordination polymer signal amplification technology, the specific steps are as follows:

[0036] (1) Dispersion of graphene

[0037] Dissolve 8.0 mg of graphene in 8.0 mL of acetic acid buffer solution with a concentration of 0.2 M and pH=5.0, and ultrasonically disperse in an ultrasonic cleaner for 3.5 hours to obtain a graphene dispersion;

[0038] (2) Preparation of nucleic acid-like CoA-Au(I) coordination polymer

[0039] Sequentially pipette 5.0 μL of 30 μM HAuCl 4 ·3H 2 O, 15.0 μL of 80 μM CoA solution, 70.0 μL of 5 mM phosphate buffer solution with a pH of 5.0 in a PCR tube, oscillating and reacting on a constant temperature magnetic stirrer at 30°C for about 10 minutes. The color of the reaction solution changed from yellow to colorless, and the nucleic acid-like CoA-Au(I) coordination polymer was obtained.

[0040] (3) Preparation of elec...

Embodiment 2

[0044] A method for preparing an electrochemical biosensor based on the analog peroxidase activity-like nucleic acid CoA-Au(I) coordination polymer signal amplification technology, the specific steps are as follows:

[0045] (1) Dispersion of graphene

[0046] Dissolve 5.0 mg of graphene in 5.0 mL of acetate buffer solution with a concentration of 0.1 M and pH=5.5, and ultrasonically disperse in an ultrasonic cleaner for 2 hours to obtain a graphene dispersion;

[0047] (2) Preparation of nucleic acid-like CoA-Au(I) coordination polymer

[0048] Sequentially pipette 8.0 μL of 40 μM HAuCl 4 ·3H 2 O, 10.0 μL of 90 μM CoA solution and 80.0 μL of 10 mM phosphate buffer solution with a pH of 7.0 were placed in a PCR tube, and oscillated on a constant temperature magnetic stirrer at 30°C for about 8 minutes. The color of the reaction solution changed from yellow to colorless, and the nucleic acid-like CoA-Au(I) coordination polymer was obtained.

[0049] (3) Preparation of elect...

Embodiment 3

[0053] A method for preparing an electrochemical biosensor based on the analog peroxidase activity-like nucleic acid CoA-Au(I) coordination polymer signal amplification technology, the specific steps are as follows:

[0054] (1) Dispersion of graphene

[0055] 7.0mg graphene is dissolved in 8.0mL concentration and is the acetate buffer solution of 0.1M pH=6.0, ultrasonically disperses 4h in ultrasonic cleaner, obtains graphene dispersion liquid;

[0056] (2) Preparation of nucleic acid-like CoA-Au(I) coordination polymer

[0057] Sequentially pipette 15.0 μL of 60 μM HAuCl 4 ·3H 2 O, 15.0 μL of 100 μM CoA solution and 90.0 μL of 15 mM phosphate buffer solution with a pH of 8.0 were placed in a PCR tube, and oscillated on a constant temperature magnetic stirrer at 30°C for about 10 minutes. The color of the reaction solution changed from yellow to colorless, and the nucleic acid-like CoA-Au(I) coordination polymer was obtained.

[0058] (3) Preparation of electrochemical bi...

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Abstract

The invention discloses a preparation method and application of an electrochemical biosensor based on a nucleic acid-like CoA-Au(I) coordination polymer capable of simulating peroxidase character. The preparation method comprises the following specific steps: (1) dissolving graphene into an acetic acid buffer solution and ultrasonically dispersing in an ultrasonic cleaner to obtain graphene dispersion liquid; (2) evenly mixing coenzyme A, chloroauric acid and a phosphoric acid buffer solution in a PCR pipe, continuously oscillating to enable gold ions to be reduced and obtaining a CoA-Au(I) coordination polymer in room temperature; (3) electrolytically depositing graphene on a bare glassy carbon electrode and then dropwise adding a CoA-Au(I) coordination polymer solution to be used for detecting hydrogen peroxide and hydrogen peroxide concentration in a biological sample. The electrochemical biosensor has the advantages of good specificity, high flexibility, quick detection speed, accurate and reliable result and low cost.

Description

technical field [0001] The present invention relates to an electrochemical biosensor, in particular to a preparation method of an electrochemical biosensor for catalyzing the reduction of hydrogen peroxide by a nucleic acid-like coenzyme A-Au(I) coordination polymer signal amplification technology simulating the properties of peroxidase and its application in Detection applications in biological environments. Background technique [0002] As an efficient biocatalyst, enzymes in nature can effectively participate in most reactions. Due to its high efficiency, high specificity and mild reaction conditions, enzyme-catalyzed reactions are widely used. However, inconvenient storage and use, difficult purification, high cost and instability limit the application of enzymes in some fields. Therefore, in recent years, researchers have begun to pay more and more attention to and study the preparation of mimetic enzymes, so that they have similar functions to natural enzymes. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48
Inventor 胡宇芳张青青
Owner NINGBO UNIV
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