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Streptococcus salivarius and application thereof in preparing ozostomia eliminating drug

A technology for streptococcus salivarius and halitosis, applied in the field of microorganisms, to achieve the effect of wide application prospects and value

Active Publication Date: 2017-08-18
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researchers proposed that S. salivarius K12 can be used as an effective adjuvant treatment in the treatment of halitosis, but long-term and periodic intake of preparations containing S. salivarius K12 is required to inhibit oral odor-producing bacteria

Method used

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  • Streptococcus salivarius and application thereof in preparing ozostomia eliminating drug
  • Streptococcus salivarius and application thereof in preparing ozostomia eliminating drug
  • Streptococcus salivarius and application thereof in preparing ozostomia eliminating drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The screening of embodiment 1 bacterial strain

[0020] Implementation goal: To find natural bacteria that can inhibit halitosis by isolating the oral dominant flora from the saliva of 250 children without halitosis and detecting the decomposition of sulfur-containing compounds.

Embodiment approach

[0022] (1) Sampling: 250 normal children aged 4-7 years, without bad breath, without oral diseases, without family genetic diseases, without systemic diseases, and without long-term medication history. Before saliva collection, the purpose and process of the experiment were explained to the school and parents, and consent was obtained. Children should not eat or drink within 2 hours after breakfast. Use a 15ml sterile sample collection tube to naturally collect about 2ml of saliva without external stimulation of saliva secretion. Each sample is divided into three tubes, which are used for PCR identification, bacterial culture and storage respectively.

[0023] (2) Separation and culture: All samples were separated and cultured, diluted with PBS, evenly spread on the brain heart infusion BHI culture dish, placed at 37°C, 5% CO 2 incubator for cultivation.

[0024] (3) Identification by matrix-assisted laser desorption tandem time-of-flight mass spectrometry (MALDI-TOF MS):

...

Embodiment 2

[0087] Embodiment 2. The use and effect of bacterial strains preventing and treating halitosis

[0088] Detection of the ability of dominant Streptococcus salivarius to decompose volatile sulfide produced by standard strains of bad breath:

[0089] By detecting the concentration of hydrogen sulfide and methyl mercaptan in the gas above the co-culture solution of Clostridium nucleatum and dominant Streptococcus salivarius. Gas chromatography was used to detect the sulfur-containing gas concentrations of hydrogen sulfide and methyl mercaptan in the upper gas after co-cultivation of Clostridium nucleatum and dominant Streptococcus salivarius. The results showed that there were higher concentrations of hydrogen sulfide and methyl mercaptan ( Figure 4 ), while the concentration of hydrogen sulfide decreased by 64.6% and the concentration of methyl mercaptan decreased by 71.9%% ( Figure 5 ).

[0090] Table 1 is the specific detection value

[0091] Table 1. HPLC detection of s...

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Abstract

The invention provides an oral probiotics strain, the preservation number of which is CGMCC NO.12899, the preservation data of which is August 23, 2016, the preservation class of which is named as Streptococcus Salivarius, and the preservation unit of which is CGMCC with the address of Institute of Microbiology, CAS, #3, Yard #1, West Beichen Road, Chaoyang District, Beijing. The strain can obviously inhibit generation of volatile sulfide compounds of an ozostomia standard strain with clostridia. The invention also discloses an application of the strain in preparing the ozostomia eliminating drug, and the strain has wide application prospect and value in eliminating ozostomia and preparing a microecological preparation for establishing a beneficial oral microecological environmental effect.

Description

technical field [0001] The invention relates to a probiotic and its application, belonging to the field of microorganisms. Background technique [0002] Halitosis refers to a symptom of bad breath when breathing, which is a common disease, and the prevalence rate of Chinese people varies from 22% to more than 50%. Halitosis patients dare not communicate with others in close quarters, thereby producing inferiority complex, affecting normal interpersonal and emotional communication, which is very distressing. Bad breath is mainly caused by bacteria in the oral cavity, especially the volatile sulfide (Volatile Sulfur Compound, VSC) produced by the fermentation of Gram-negative anaerobic bacteria gathered in the back 1 / 3 of the tongue. Studies have shown that VSC is mainly produced by bacteria such as Porphyromonas gingivalis, Steiner forsythium, Prevotella intermedia and Fusobacterium nucleatum. Kazor et al. used PCR to analyze the bacteria in the oral cavity of healthy peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/744A61P1/02C12R1/46
CPCA61K35/744A23V2002/00C12R2001/46C12N1/205A23V2400/245A23V2200/30
Inventor 孙丽娜徐建国李振军
Owner ICDC CHINA CDC
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