Bacillus amyloliquefaciens RH9, as well as screening method and applications thereof

Abstract

The invention discloses bacillus amyloliquefaciens RH9, the bacillus amyloliquefaciens RH9 is preserved in the China General Microbiological Culture Collection Center (CGMCC), is assigned with the accession number of CGMCC 12489, and has high yield of iturinA, and iturinA produced by bacillus amyloliquefaciens RH9 has very good application prospects in the agriculture, industry and environmental protection. The invention further discloses a screening method of the bacillus amyloliquefaciens RH9. The screening method comprises the following steps: activating the original strains; carrying out penicillin mutation, thus obtaining a first positive mutation strain and a second positive mutation strain; carrying out nitrosoguanidine mutation, thus obtaining a third mutation strain and a fourth mutation strain; carrying out genome shuffling on the positive mutation strains, thus obtaining the first round of genome shuffling strains with high yield of iturinA; carrying out genome shuffling on the first round of genome shuffling strains, thus obtaining the second round of genome shuffling strains with high yield of iturinA, and selecting one of the second round of genome shuffling strains which has high yield of iturinA and high hereditary stability, wherein the selected genome shuffling strain is named bacillus amyloliquefaciens RH9.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus amyloliquefaciens RH9, a screening method and application. Background technique [0002] Antimicrobial peptides have a strong killing effect on a variety of Gram-positive and negative bacteria in food, and can quickly inhibit the growth of microorganisms. After eating, they are easily hydrolyzed and digested by proteases in the body and have no toxic side effects. They are very active under acidic conditions. Strong, with good solubility and stability, it is a new type of food preservative with great development prospects. [0003] Iturin A (iturinA) and its homologues are also antimicrobial peptides, mainly derived from the secondary metabolites of Bacillus strains. They have the advantages of broad-spectrum disease resistance and are not easy to produce drug resistance. High inhibitory activity, especially strong antifungal activity. [0004] Bac...

AI Technical Summary

Problems solved by technology

But in practice, it has not been widely used in industry and environmental protection. The reason is that its production cost is huge, and it lacks a high-yiurinA-producing Bacillus amyloliquefaciens

Images