Vascularized tissue-engineered bone without heterologous scaffold and preparation method thereof

Abstract

The invention relates to vascularized tissue-engineered bone without heterologous scaffold and a preparation method thereof; the vascularized tissue-engineered bone without heterologous scaffold comprises an upper endothelial angioblast film and a lower osteoblast film; rabbit adipose-derived stem cells feature potential multilineage differentiation and are induced to differentiate, and the double cell films able to form bone and vessels are prepared and are used to construct the vascularized tissue-engineered bone. The source of cells is rich, separation and acquisition are easy, little donor injury is caused, and culture requirements are low; both osteoblast and endothelial angioblast required to construct the vascularized tissue-engineered bone are from adipose-derived stem cells, and adding endothelial cells of different sources is not required; self-secreted extracellular matrix of the cells is used as an 'endogenous scaffold', and body immunologic rejection and inflammatory reaction are avoided; the preparation method is simple, has mild conditions and has good osteogenic and angioblastic properties.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a vascularized tissue-engineered bone without exogenous support constructed by an osteoblast membrane sheet and an angioblast endothelial cell membrane sheet and a preparation method thereof. Background technique [0002] The treatment of large bone defects caused by severe trauma, tumor resection, infection, and congenital deformities is a difficult problem and a huge challenge for modern medicine. At present, the commonly used repair methods in clinic include autologous bone graft, allogeneic bone graft and the use of artificial bone, etc., but the above methods all have certain defects. Autologous bone grafting is recognized as the gold standard for bone tissue repair, but patients have to endure the trauma of autologous tissue transplantation surgery, and the donor area is limited. Therefore, autologous bone grafting cannot be regarded as an ideal repair method for large-...

AI Technical Summary

Problems solved by technology

However, the disadvantage of the traditional "cell-scaffold" strategy is that when the seed cells are planted on the scaffold, 30-40% of the cells cannot adhere to the scaffold and are lost, and the cell adhesion rate is low and the utilization rate is poor; There are few interactions between cells, and it is difficult to form the proper microenvironment for cells in the body. After the cell-scaffold complex is implanted in the body, bone formation always occurs at the periphery of the scaffold, which affects the size and quality of osteogenesis.

Although there have been many studies on the construction of vascularized tissue-engineered bone, the strategy of using adipose-derived osteoblasts and endothelial cells to prepare bicellular composite membranes with osteogenic and angiogenic capabilities to construct vascularized tissue-engineered bone is still unknown. few people involved

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