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Preparation method of adipose gel enriched in adipose-derived stem cell and extracellular matrix

An adipose stem cell and extracellular matrix technology, applied in the field of fat transplantation and stem cell therapy, can solve the problems of complexity, high cost, inability to remove fat cells and oil, etc., and achieve the effect of simple operation, thorough fragmentation, and guaranteed rigor

Inactive Publication Date: 2017-08-18
王大鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Yet prior art is comparatively complicated in the process of obtaining fat gel, and cost is higher, can not reach good effect for the extraction of fat gel, especially can not remove mature adipocyte and grease, therefore, can not provide for fat transplantation. better material

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0030] The present invention aims to provide a method for preparing fat gel rich in fat stem cells and extracellular matrix, comprising the following steps:

[0031] 1) Use a threaded syringe to extract fat particles for transplantation, add heparin sodium to the extracted fat particles, and mix the threaded syringe upside down;

[0032] 2) Put the above-mentioned fat particles into the first centrifuge tube and centrifuge at 1200g / min for 3 minutes. After centrifugation, the fat particles are divided into upper and lower layers. After removing the swelling liquid in the lower layer, transfer the fat particles in the upper layer to the first in the syringe;

[0033] 3) Connect the threaded syringe containing fat particles with the second syringe through the fat breaker, push the piston of the threaded syringe containing fat particles, and push the fat particles into the first syringe on the opposite side through the fat breaker, The injection speed is 5-10ml / s, so repeat the ...

Embodiment 1

[0088] A method for preparing fat gel rich in fat stem cells and extracellular matrix, comprising the following steps:

[0089] 1) Use a threaded syringe to extract fat particles for transplantation, add heparin sodium to the extracted fat particles, the concentration of the heparin sodium is 10U / ml, turn the threaded syringe up and down to mix it;

[0090] 2) Put the above-mentioned fat particles into the first centrifuge tube and centrifuge at 1200g / min for 3 minutes. After centrifugation, the fat particles are divided into upper and lower layers. After removing the swelling liquid in the lower layer, transfer the fat particles in the upper layer to the first in the syringe;

[0091]3) Connect the threaded syringe containing fat particles with the second syringe through the fat breaker, push the piston of the threaded syringe containing fat particles, and push the fat particles into the first syringe on the opposite side through the fat breaker, The injection speed is 5-10m...

Embodiment 2

[0098] A method for preparing fat gel rich in fat stem cells and extracellular matrix, comprising the following steps:

[0099] 1) Use a threaded syringe to extract fat particles for transplantation, add heparin sodium to the extracted fat particles, the concentration of the heparin sodium is 10U / ml, turn the threaded syringe up and down to mix it;

[0100] 2) Put the above-mentioned fat particles into the first centrifuge tube and centrifuge at 1200g / min for 3 minutes. After centrifugation, the fat particles are divided into upper and lower layers. After removing the swelling liquid in the lower layer, transfer the fat particles in the upper layer to the first in the syringe;

[0101] 3) Connect the threaded syringe containing fat particles with the second syringe through the fat breaker, push the piston of the threaded syringe containing fat particles, and push the fat particles into the first syringe on the opposite side through the fat breaker, The injection speed is 5-10...

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Abstract

The invention discloses a preparation method of adipose gel enriched in an adipose-derived stem cell and an extracellular matrix. A mature adipose cell and the extracellular matrix in an adipose transplanting material are crushed through a physical cutting method; the filtration and the emulsification are carried out by using an adipose emulsifier, so as to remove an uncrushed component in adipose and obtain chylous adipose; by adopting a flocculation precipitation technique, grease in the chylous adipose is collected and removed by using polyethylene glycol, so as to obtain the adipose gel rich in the adipose-derived stem cell and the extracellular matrix. According to the adipose gel obtained by the method, as the mature adipose cell and the grease are removed, the volume of original adipose is concentrated for approximately 10 times; the content of the adipose-derived stem cell and the extracellular matrix is far higher than that of common adipose; a favorable material is provided for the transplantation and filling of the adipose.

Description

technical field [0001] The invention belongs to the fields of fat transplantation and stem cell therapy, and in particular relates to a method for preparing fat gel rich in fat stem cells and extracellular matrix. Background technique [0002] The application of adipose stem cells and stromal vascular fragment cells in the field of stem cell therapy is becoming more and more extensive. In particular, the application of stromal vascular fragment cells has been carried out in clinical trials in many countries. It has obvious advantages in improving the preservation rate and wound repair after fat transplantation: 1. The adipose stem cells freshly isolated from the tissue contain a large number of biologically active autologous adipose stem cells; 2. It is derived from its own adipose tissue without immune rejection; 3. It has abundant reserves in the human body; 4. No need for in vitro culture, reducing bacterial contamination risks of. [0003] Yet prior art is comparatively...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50
CPCA61L27/3604A61L27/3633A61L27/3641A61L27/3834A61L27/50A61L2430/34
Inventor 王大鹏
Owner 王大鹏
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