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Use of Russula polysaccharide from gray meat in the preparation of medicine for treating systemic lupus erythematosus

A technology of Russula polysaccharide and lupus erythematosus, which is applied in the field of medicine, can solve the problems such as the medical use document report of lupus erythematosus disease, which has not been seen, and achieves the effects of significant curative effect and small toxic and side effects.

Inactive Publication Date: 2019-11-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no document report on the medical use of gray meat russula polysaccharide in the treatment of systemic lupus erythematosus

Method used

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  • Use of Russula polysaccharide from gray meat in the preparation of medicine for treating systemic lupus erythematosus
  • Use of Russula polysaccharide from gray meat in the preparation of medicine for treating systemic lupus erythematosus
  • Use of Russula polysaccharide from gray meat in the preparation of medicine for treating systemic lupus erythematosus

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The preparation method of the crude polysaccharide of the fruit body of gray meat russula russula comprises the following steps in sequence:

[0030] 1) Dry the fruiting body of the freshly picked gray meat russula until the quality does not change, and completely remove the water;

[0031] 2) Use a pulverizer to crush and dry the fruiting bodies, and pass through a 100-mesh sieve to remove larger particles;

[0032] 3) Weigh 40 g of russula fruiting body powder, add 1L of ultrapure water, and extract in a water bath at 90°C for 3 h. Filter the extract with double-layer filter paper, and the obtained filtrate is the extract, and the extract is concentrated to 50 mL with a rotary evaporator;

[0033] 4) Slowly add absolute ethanol 4 times the volume of the concentrated solution to the concentrated extract and mix well,

[0034] Stand at 40°C for 12-16 h, retain the precipitate, wash thoroughly with 75% ethanol, and freeze-dry;

[0035] 5) Resuspend and dissolve the pr...

Embodiment 2

[0039] The method for separating and purifying polysaccharide PRG1-2 from the fruiting bodies of russula russula russula follows the following steps in sequence:

[0040] 1) Ion exchange chromatography: Resuspend the lyophilized powder of Russula russula crude polysaccharide in ultrapure water, and filter with a 0.22 μM filter membrane. Equilibrate the DEAE-52 column with ultrapure water in advance, slowly put the crude polysaccharide solution on the column at a speed of 1mL / min, wash the column with 2 times the column volume of ultrapure water, and then use 0.1M NaCl, 0.2 M NaCl and 0.3M NaCl respectively The aqueous solution eluted the column at a flow rate of 1.0mL / min, and collected all the eluted polysaccharide samples, and eluted the sample PRG1 with 0.2 M NaCl for the next step of purification;

[0041] 2) The PRG1 in the previous step was subjected to molecular sieve separation and purification. First, equilibrate sephadex G100 with 0.2 M NaCl aqueous solution, load P...

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Abstract

The invention discloses a medical usage of russula griseocarnosa polysaccharide in treatment of systemic lupus erythematosus. The russula griseocarnosa polysaccharide PRG1-2 has the advantages of adjusting the activity of T cell subset, reducing IL-10, ds-DNA antibody and urine protein, being obviously effective in treatment, safe and reliable. Provided is an application of the russula griseocarnosa polysaccharide PRG1-2 with a wide clinical application prospect in preparation of medicine for treating systemic lupus erythematosus.

Description

technical field [0001] The invention discloses the medical application of russula polysaccharide in treating systemic lupus erythematosus, provides the new medical application of russula polysaccharide, and belongs to the technical field of medicine. Background technique [0002] Systemic Lupus Erythematosus (SLE) is a common clinical autoimmune disease characterized by autoantibody production, complement activation and immune complex deposition. The main feature of SLE pathogenesis is abnormal autoimmune tolerance, so immunological factors have been widely valued. Studies have shown that CD3+CD4+T cells in the peripheral blood of SLE patients decreased, CD3+CD8+ increased, and IL-10 levels were up-regulated. IL-10 has both immunostimulatory and immunosuppressive effects. Studies have shown that IL-10 can stimulate PBMCs of patients with systemic lupus erythematosus to produce anti-dsDNA antibodies, and the level in SLE patients is significantly higher than that of healthy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P37/02A61P19/04C08B37/00
CPCA61K31/715A61K2236/15A61K2236/331A61K2236/53A61K2236/55C08B37/0003
Inventor 孟兆丽陈倩初晓
Owner JILIN UNIV
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