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Novel arsenic aptamer nucleic acid sequence, and applications thereof in detection of arsenic ions

A technology of nucleic acid sequence and aptamer, which is applied in the measurement of color/spectral characteristics, analysis by making materials react chemically, and material analysis by observing the influence on chemical indicators, etc. It can solve the problem of single reagents and operating conditions, etc. problems, to achieve the effect of simple synthesis, low instrument cost and reagent cost, and low cost

Inactive Publication Date: 2017-08-15
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The present invention aims to solve the above-mentioned problems, and the purpose is to provide a novel arsenic aptamer nucleic acid sequence and the application of detecting arsenic ions, so as to overcome the single reagents and operating conditions for using aptamers to detect arsenic ions. insufficient

Method used

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  • Novel arsenic aptamer nucleic acid sequence, and applications thereof in detection of arsenic ions
  • Novel arsenic aptamer nucleic acid sequence, and applications thereof in detection of arsenic ions
  • Novel arsenic aptamer nucleic acid sequence, and applications thereof in detection of arsenic ions

Examples

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Effect test

Embodiment

[0022] The nucleic acid sequences used in this example were chemically synthesized using the known classic method, the phosphoramidite triester method. Its sequence is as follows:

[0023] ACA GAA CAA CCAACG TCG CTC CGG GTA CTT CTT C

[0024] 1. Preparation of gold nanoparticles

[0025] Utilize sodium citrate reduction method to synthesize nano-gold, get 50mL 1mmol / L tetrachloroauric acid solution and pour into double-necked bottle, put into oil bath pan and heat, through 400r / min magnetic stirring, when observing the When the condensed water starts to reflux, quickly add 5mL of sodium citrate. At this time, the color of the solution changes rapidly, from light yellow to colorless, then to brownish black, and finally to wine red. Continue to heat and reflux for 5 minutes, remove the heat source, but continue to stir, let the reaction system cool down to room temperature naturally, and obtain nano-gold AuNPs.

[0026] 2. Preparation of standard substance solution

[0027] ...

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Abstract

The invention discloses a novel arsenic aptamer nucleic acid sequence. The novel arsenic aptamer nucleic acid sequence is ACA GAA CAA CCA ACG TCG CTC CGG GTA CTT CTT C. The novel arsenic aptamer nucleic acid sequence is relatively short; synthesis is simple; cost is low; and auxiliary reagents such as nanogold and sodium chloride are easily available. In detection of arsenic ions, colorimetric method is adopted, a cheap spectrophotometer can be used for detection, equipment cost and reagent cost are both lower than that of conventional Ars-3 aptamer detection system, and the novel arsenic aptamer nucleic acid sequence is more suitable for rapid detection.

Description

technical field [0001] The invention belongs to the field of environmental safety monitoring, and in particular relates to a nucleic acid sequence for colorimetric detection of arsenic ion concentration. Background technique [0002] Arsenic ion is a heavy metal ion. Arsenic has a strong toxic effect on animals and plants. Due to the increasing pollution of arsenic in soil, water and crops, it has become a common environmental poison and has attracted widespread attention worldwide. For the detection of heavy metals, a variety of detection methods have been developed, such as atomic absorption spectrometry, fluorescence method, electrochemical method, biosensor method and other methods. At present, there are chemical methods and instrument methods for the detection of arsenic. The chemical methods include the arsenic spot method and the silver salt method. Because the chemical method is cumbersome and the sensitivity is not high, it does not meet the needs of modern developm...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
Inventor 袁敏宋志宏徐斐曹慧于劲松叶泰
Owner UNIV OF SHANGHAI FOR SCI & TECH
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