Bacillus amyloliquefaciens HMB28388 and application thereof
A technology of HMB28388 and amylolytic spores, applied in application, bacteria, fungicides, etc., can solve the problems of new diseases or secondary diseases, difficulty in cultivating resistant varieties, less understanding of resistant materials, etc., and achieve high efficacy , It is not easy to produce drug resistance, and the effect of drug durability is good
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Embodiment 1
[0050] The preparation of embodiment 1HMB28388 microbial bacterial agent
[0051] Follow the steps below:
[0052] (1) Strain activation: The Bacillus amyloliquefaciens strain HMB28388 stored at -80°C (the strain HMB28388 had been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 28, 2016, and the preservation number was CGMCC No. .13210) was activated (30°C) on LB plate culture medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), pick A single colony was cultured at 30°C for 30 hours on LB slant medium (its composition and weight ratio: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) to obtain activated the strain;
[0053] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium ch...
Embodiment 2
[0056] Embodiment 2 Antagonism test of HMB28388 bacterial strain of the present invention to cucumber target spot bacterium
[0057] (1) Source of Cucumber target spot fungus CA-1 strain: Cucumber target spot spot fungus CA-1 strain was collected from cucumber diseased leaves in Da Da Village, Mancheng County, Baoding City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Department of Plant Pathology, School of Plant Protection, Hebei Agricultural University identified it as Corynespora cassiicola, and the pathogenicity test showed strong pathogenicity.
[0058] (2) Test method:
[0059] Plate stand-off experiment: First, activate and culture the CA-1 strain of Cucumber spp. The sheet is transferred in the center of another PDA plate; the Bacillus amyloliquefaciens HMB28388 activated in the step (1) of Example 1 is connected to 2.0 centimeters away from the indicator bacteria sheet, an...
Embodiment 3
[0064] Example 3 Comparison test of the control effect of HMB28388 bacterial strain of the present invention on cucumber target spot disease
[0065] (1) Test treatment:
[0066] (1) HMB28388 microbial bacterial agent: 100 times water dilution of the HMB28388 liquid preparation prepared in Example 1;
[0067] (2) Blank control: clear water.
[0068] (2) Test method:
[0069] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.
[0070] (1) Cultivate cucumber plants: Cultivate Jinyou No. 1 cucumber seedlings in a nutrient bowl with a diameter of 6 cm. The seedling substrate is vermiculite. Two seeds are sown in each nutrient bowl, and one strong seedling is retained after emergence. When the cucumber seedlings grow two true leaves, they are transplanted into plastic flower pots with a diameter of 25 cm (the flower pots are filled with a substrate made of vermic...
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