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Bacillus amyloliquefaciens HMB28353 and application thereof

A technology for dissolving starch spores and bacilli, applied in the application, bacteria, fungicides and other directions, can solve the problems of new or secondary diseases, pollution of agricultural products and environment, less understanding of resistant materials, etc. Highly targeted and durable

Active Publication Date: 2017-08-15
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the control methods for cucumber target spot disease include: (1) Breeding disease-resistant varieties: cultivating disease-resistant varieties is the most economical and effective method for disease control, but due to the resistance to cucumber target spot disease in domestic cucumber germplasm resources Little is known, it is more difficult to breed disease-resistant varieties
(2) Prevention and control of agricultural cultivation measures: such as reducing planting density, timely cleaning of sick and old plants and leaves, using drip irrigation under mulch to reduce greenhouse humidity, etc., but the control effect is poor
However, the long-term use of chemical pesticides in large quantities will easily cause pathogenic bacteria to develop drug resistance, resulting in reduced control effects; at the same time, it will easily cause agricultural products and environmental pollution, causing new diseases or secondary diseases to occur

Method used

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  • Bacillus amyloliquefaciens HMB28353 and application thereof
  • Bacillus amyloliquefaciens HMB28353 and application thereof
  • Bacillus amyloliquefaciens HMB28353 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1HMB28353 microbial bacterial agent

[0050] Follow the steps below:

[0051] (1) Strain activation: the Bacillus amyloliquefaciens strain HMB28353 stored at -80°C (this strain has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 28, 2016, and the preservation number is CGMCCNo.13209 ) on LB plate medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) to activate (30 ℃), pick a single colony In LB slant medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) at 30 ℃ for 24 to 36 hours to obtain activated strains.

[0052] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 1000mL), i...

Embodiment 2

[0055] Embodiment 2 Antagonism test of HMB28353 bacterial strain of the present invention to cucumber target leaf spot bacterium

[0056] (1) Source of Cucumber target spot bacteria strain CA-1: Cucumber target spot bacteria CA-1 strain was collected from cucumber diseased leaves in Da Da Village, Mancheng County, Baoding City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Department of Plant Pathology, School of Plant Protection, Hebei Agricultural University identified it as Corynespora cassiicola, and the pathogenicity test showed strong pathogenicity.

[0057] (2) Test method:

[0058] Plate confrontation experiment: firstly, the CA-1 strain of target leaf spot of cucumber was activated and cultured on a PDA plate for 7 days, and then the Punch holes in the colony edge area to make bacterial flakes, transfer the cucumber target spot bacteria flakes to another PDA plate center, t...

Embodiment 3

[0063] Example 3 Comparison test of HMB28353 bacterial strain of the present invention on the control effect of cucumber target spot disease

[0064] (1) Test materials:

[0065] (1) Cucumber variety: Jinyou No. 1.

[0066] (2) Spore suspension of target leaf spot fungus CA-1 (the source of target spot fungus CA-1 is the same as in Example 2).

[0067] (2) Test treatment:

[0068] (1) HMB28353 liquid preparation: a 100-fold water dilution of the HMB28353 liquid preparation prepared in Example 1.

[0069] (2) Blank control: clear water.

[0070] (3) Test method:

[0071] (1) Cultivate cucumber plants: Cultivate Jinyou No. 1 cucumber seedlings in a nutrient bowl with a diameter of 6 cm. The seedling substrate is vermiculite. Two seeds are sown in each nutrient bowl, and one strong seedling is retained after emergence. When the cucumber seedlings grew two true leaves, they were transplanted into plastic flower pots with a diameter of 25 cm (the flower pots were filled with a...

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Abstract

The invention discloses a bacillus amyloliquefaciens strain HMB28353, the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 13209. The invention also discloses a microbial agent produced by using the bacillus amyloliquefaciens HMB28353. The bacillus amyloliquefaciens HMB28353 has high antibacterial effect on cucumber target leaf spot, and the average control effect is above 90.0%; also, the bacillus amyloliquefaciens HMB28353 is not easy to generate resistance in controlling cucumber target leaf spot, and the persistence of the antibacterial effect is good; in addition, the bacillus amyloliquefaciens HMB28353 does not have the problem of environmental pollution; and the HMB28353 strain also has the characteristic of wide antibacterial spectrum, and besides cucumber target leaf spot bacteria, the HMB28353 strain also has good inhibiting effects on verticillium dahliae kleb, fusarium oxysporum f. sp. vasinfectum, cotton rhizoctonia solani or botrytis cinerea pers.

Description

technical field [0001] The invention belongs to the field of biological control, and specifically relates to a bacillus amyloliquefaciens HMB28353, and also relates to microbial agents produced by the bacillus amyloliquefaciens, and their application in preventing and treating plant diseases such as cucumber target spot disease. Background technique [0002] Cucumber target spot disease (also known as Corynespora leaf spot, brown spot, "yellow spot disease", etc.) is caused by Corynespora cassiicola (Berk & Curt) Wei., especially in wintering greenhouses, winter-spring greenhouses, Serious occurrences occurred in protected areas such as spring greenhouses. The symptoms are yellow water-soaked spots with a diameter of about 1 mm at the beginning; in the middle stage of the disease, the lesions expand to round or irregular shapes, and are easy to perforate. The lesions on the front of the leaves are rough and uneven. The diameter of the lesion can reach 10-15 mm, and there is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 李社增马平鹿秀云张晓云郭庆港丛蓉王培培赵卫松张丽红
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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