Application of tuberculosis microbial marker in preparation of reagents for diagnosing tuberculosis

A technology for tuberculosis and microorganisms, which is applied in the field of application of tuberculosis microbial markers in the preparation of reagents for diagnosing tuberculosis, and can solve problems such as no research on tuberculosis intestinal flora biomarkers

Pending Publication Date: 2017-08-11
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Currently, no studies have addressed TB-associated gut microbiota biomarkers

Method used

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  • Application of tuberculosis microbial marker in preparation of reagents for diagnosing tuberculosis
  • Application of tuberculosis microbial marker in preparation of reagents for diagnosing tuberculosis
  • Application of tuberculosis microbial marker in preparation of reagents for diagnosing tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1: Sample collection

[0074] All 20 stool samples were collected from 20 volunteers respectively, and the stool sample collection was performed by the Sixth People's Hospital of Dongguan, China. Diagnosed tuberculosis patients were used as the case group, and other non-diabetic individuals were used as the control group.

Embodiment 2

[0075] Example 2: DNA extraction and sequencing

[0076] 2.1 Storage of stool samples

[0077] Put the collected stool samples into sterilized stool collection tubes, store them in the freezer immediately, and add glycerol to the stool samples to freeze. Frozen samples were sent to the storage point and stored at -80°C until use.

[0078] 2.2 DNA extraction

[0079] 200 mg of frozen fecal samples were taken from each part and extracted with QIAGEN Fecal DNA Extraction Kit.

Embodiment 3

[0080] Example 3: Identification of biomarkers

[0081] refer to figure 1 and figure 2 The experimental process for the determination and identification of tuberculosis biomarkers.

[0082] 3.1 Basic processing of sequencing data

[0083] EBI raw data has been quality controlled and de-hosted, and the data processing steps are as follows:

[0084] (1) Remove adapter-contaminated Reads (the number of adapter-contaminated bases in Reads is greater than 5 bp);

[0085] (2) Remove low-quality Reads (more than 15% of Reads with a base quality value lower than 19);

[0086] (3) Remove the Reads containing more than 5% N.

[0087] 3.2 Obtaining TB microbiome gene sets

[0088] The main body of metagenomic biomarkers is genes and corresponding functions, so it is necessary to assemble sequenced sequences and predict genes, remove redundancy, and construct a non-redundant reference gene set. Use SOAPdonovo, Velvet and SPAdes short-sequence assembly software to construct images ...

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Abstract

The invention belongs to the field of biomedicine and relates to application of a tuberculosis microbial marker in the preparation of reagents for predicting or assisting in diagnosing tuberculosis state of a subject. The invention also discloses a method of using a tuberculosis marker to determine the state of an individual. The tuberculosis microbial marker claimed herein provides significant differences between tuberculous patients and healthy ones, may act as a marker to distinguish healthy people and tuberculous patients, and may predict, diagnose or prognose tuberculosis.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to tuberculosis microbial markers and applications thereof, more specifically, the present invention relates to the application of tuberculosis microbial markers in the preparation of reagents for predicting or assisting the diagnosis of tuberculosis status of objects, using the tuberculosis microorganisms A system for predicting the curative effect of individual tuberculosis patients with markers, the application of said tuberculosis microbial markers in the preparation of drugs and / or functional foods for treating tuberculosis, a kit for detecting tuberculosis biomarkers, and drugs for treating tuberculosis , methods of producing or screening said medicaments, and vaccines for the treatment of tuberculosis. Background technique [0002] Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) infection is one of the severe infectious diseases with the highest infection rate,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12M1/00C12M1/34A61K45/00A61P31/06
CPCA61K45/00C12Q1/6883C12Q2600/136Y02A50/30
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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