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Bacillus siamese b-3 and its application

A technology of bacillus and bacteria agent, applied in the biological field, can solve problems such as environmental pollution, decline in control effect, and endangering human and animal health

Active Publication Date: 2019-11-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of chemical agents, on the one hand, pathogenic bacteria are likely to develop drug resistance, thereby greatly reducing the control effect; on the other hand, it is easy to cause serious environmental pollution, endangering the health of humans and animals

Method used

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  • Bacillus siamese b-3 and its application

Examples

Experimental program
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preparation example Construction

[0057] The preparation method of potato liquid medium is as follows: take 200 g of peeled potatoes, cut them into small pieces, add 1.0 L of distilled water, and boil for 30 minutes; collect the filtrate after gauze filtration, add 20.0 g of glucose to the filtrate, and distill the volume to 1.0 L, the pH value is natural, and then sterilized at 115°C for 15 minutes.

[0058] The preparation method of potato liquid medium 1 is as follows: take 200 g of peeled potatoes, cut into small pieces, add 1.0 L of distilled water, and boil for 30 minutes; collect the filtrate after gauze filtration, add 20.0 g of glucose in the filtrate, and distill to volume with distilled water to 1.0L, adjust the pH to 4.0; then sterilize at 115°C for 15 minutes.

[0059] Potato liquid medium 2: In the preparation method of potato liquid medium 1, "adjust the pH value to 4.0" was replaced with "adjust the pH value to 5.0", and the other steps were the same.

[0060] Potato liquid medium 3: In the pr...

Embodiment 1

[0065] Example 1. Isolation, Identification and Preservation of Bacillus siamensis B-3 CGMCC No.13753

[0066] 1. Separation

[0067] 1. Processing of soil samples

[0068] (1) Add 10 g of soil sample (collected from the soil of potatoes planted in Pingjibao, Ningxia Hui Autonomous Region) and an appropriate amount of glass beads to 90 mL of sterile water, shake for 10 min, and let stand for 30 s to make a soil stock solution (at this time, the dilution is recorded as 10 -1 ). Take 1 mL of the supernatant of the soil stock solution and add it to a test tube filled with 9 mL of sterile water and mix well (the dilution is 10 at this time). -2 ), then draw 1mL from this test tube and add it to another test tube filled with 9mL sterile water and mix well, and so on to make dilution A (dilution is 10 -3 ) and diluent B (dilution of 10 -4 ).

[0069] 2. Take Streptomyces scab 4.76, inoculate it on an oat solid plate, and culture it statically at 28°C for 10 days; then add an ap...

Embodiment 2

[0082] The propagation of embodiment 2, bacillus siamese B-3

[0083] The specific steps of the Bacillus siamese B-3 propagation of embodiment 1 are as follows:

[0084] 1. Activate Bacillus siamese B-3 three times on beef juice solid medium, then pick a single colony and inoculate it in a 250mL Erlenmeyer flask containing 50mL beef juice liquid medium, at a constant temperature of 30°C and 200r / min Cultivate on a shaker for 2 days to obtain a culture solution.

[0085] 2. After completing step 1, take the culture medium in step 1, centrifuge at 12000r / min for 1min, discard the supernatant, and collect the precipitate.

[0086] 3. After completing step 2, take the precipitate in step 2 and resuspend it with 50mL sterile water to obtain a bacterial suspension (the concentration of Bacillus siamese B-3 is about 1.0×10 11 cfu / mL).

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Abstract

The invention discloses bacillus siamese B-3 and application thereof. The Bacillus siamese B-3 disclosed in the present invention has a preservation number of CGMCC No. 13753 in the General Microorganism Center of China Microbiological Culture Collection Management Committee. Experiments have shown that the inoculum obtained by culturing Bacillus siamese B‑3 can inhibit Streptomyces scabies, Streptomyces acidiscabies and / or Streptomyces albidoflavus, which cause potato scab growth, show that Bacillus siamese B-3 of the present invention and related bacterial agents can be used to prevent and treat potato scab.

Description

technical field [0001] The invention relates to the bacillus siamese B-3 and its application in the field of biotechnology. Background technique [0002] Potato scab is a typical soil-borne disease, which occurs in every potato producing area in my country. The main manifestation is the appearance of nearly round to amorphous suberized scab-like light brown lesions or plaques on the surface of potato tubers, and the texture is rough to the touch. Potato scab generally has two symptoms of reticulated lesions and cracked lesions. Usually, reticulated lesions and cracked lesions are limited to the cortex. When the disease is severe, the lesions are contiguous and cannot be stored, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, which greatly reduces the The quality and economic value, and the diseased seed potatoes can be transported to accelerate the spread of the disease. It poses a serious threat to my country's potato p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 张国青仲乃琴钞亚鹏赵盼杨敬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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