Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of neohesperidin in preparation of tumor prevention and treatment drugs

A technology of neohesperidin and tumor drugs, applied in the field of medical technology and health care products, can solve the problems of less research on physiological activity and no reports on tumor prevention, and achieve good application prospects and the effect of reducing tumor size.

Inactive Publication Date: 2017-08-11
杭州禹正医药科技有限责任公司
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research on neohesperidin at home and abroad only stays in its application as a food additive and the extraction of flavanones in citrus plants, while there are few studies on its physiological activity, and it is more important for its prevention of tumors. yes no report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of neohesperidin in preparation of tumor prevention and treatment drugs
  • Application of neohesperidin in preparation of tumor prevention and treatment drugs
  • Application of neohesperidin in preparation of tumor prevention and treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] One of the sources of neohesperidin in the present invention is obtained from the white cortex of the citrus plant Huyou, and its preparation method and results are as follows:

[0025] Preparation of pomelo white cortex macroporous resin crude extract powder: 16g of dried Huyou white cortex was ultrasonically extracted twice with 80% ethanol (solid-to-liquid ratio 1:25) (40°C), centrifuged at 4000rpm for 20min, combined twice The supernatant was evaporated to dryness on a rotary evaporator to obtain an extract, which was dissolved in ddH 2 In O, about 400mL was loaded with D101 macroporous resin, the column bed volume (BV) was 20mL, and the sample loading flow rate was 2mL / min. The D101 macroporous resin after loading the sample was first washed with water 4BV to remove impurities, and then 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% ethanol was used to elute naringin and new Hesperidin was washed with 4BV for each gradient, and the elution flow rate was 2mL / min. The 10%-6...

Embodiment 2

[0029]Example 2 Neohesperidin on APC induced by high-fat diet min / + Preventive effect on colon carcinogenesis in C57BL6 / J mice

[0030] First take the 6-week-old APC min / + C57BL6 / J male mice were crossed with wild-type C57 mice. The newborn mice were separated into cages at 3 weeks after birth, and their genes were identified. The primers for gene identification were 0033:5'-GCCATCCCTTCACGTTAG-3', 0034: 5'-TTCCACTTTGGCATAAGGC-3', 0758: 5'-TTCTGAGAAAGACAGAAGTTA-3', primers 0033 and 0034 amplify the primer fragment length is 600bp, this fragment indicates the wild-type gene, primers 0034 and 0758 amplify the primer fragment length is 340bp , this fragment indicates the APC mutant gene. Mice identified as gene mutations were randomly divided into 4 groups (control group, high-fat diet induction group, high-fat diet induction+NHP 50mg / kg, high-fat diet induction+NHP100mg / kg), 22 in each group , half male and half male. When the mice were 6 weeks old, they were first pre-admini...

Embodiment 3

[0033] Example 3 The preventive effect of neohesperidin on the occurrence of breast cancer in MMTV-PyVT mice

[0034] First, take 6-week-old MMTV-PyVT male mice and cross them with wild-type ICR mice. The newborn mice are separated into cages at 3 weeks after birth, and the gene identification is performed. The primer for gene identification is oIMR0384:5' -GGAAGCAAGTACTTCACAAGGG-3', oIMR0385: 5'-GGAAAGTCACTAGGAGCAGGG-3', oIMR8744: 5'-CAAATGTTGCTTGTCTGGTG-3', oIMR8745: 5'-GTCAGTCGAGTGCACAGTTT-3', primers oIMR8744 and oIMR8745 amplify the primer fragment length is 200bp, Indicates the wild-type gene, the length of primer oIMR0384 and oIMR0385 amplifies the primer fragment is 556bp, and this fragment indicates the MMTV-PyVT mutant gene. The female mice identified as gene mutations were randomly grouped with primers 0033 and 0034. The length of the primer fragment was 600bp, which indicated the wild-type gene, and the length of the primer 0034 and 0758 amplification primer fragme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of neohesperidin in the preparation of tumor prevention drugs. Pharmacological experiment researches show that the neohesperidin can significantly reduce the number of spontaneous colorectal cancer lesions in APC min / + mice and significantly change the composition proportion of intestinal microflora in mice without affecting the blood lipid level in mice or affecting the proliferation and the apoptosis of intestinal tumor cells. The neohesperidin has a good prevention and treatment effect on the occurrence of intestinal tumors, and is expected to be developed into new tumor prevention drugs or healthcare products.

Description

technical field [0001] The invention belongs to the field of medical technology and health care products, and in particular relates to the application of neohesperidin, a flavonoid compound from natural sources, or other compositions in the prevention and treatment of tumors, in particular to the application of neohesperidin in the prevention and treatment of intestinal tumors and breast cancer. The application of drugs or compound drugs or health products. Background technique [0002] Cancer is the most common malignant disease affecting human health, and it has become a disease that threatens human life and health and cannot be ignored. In my country, with the continuous improvement of people's living standards, changes in eating habits and dietary structure, and population aging, the incidence and mortality of tumors have maintained an upward trend. Among them, the incidence of colon cancer has increased significantly. Most patients When it is discovered, it is already i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048
Inventor 杨波何俏军曹戟应美丹董蓉朱虹
Owner 杭州禹正医药科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com