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Aie luminogens for bacteria imaging, killing, photodynamic therapy and antibiotics screening, and their methods of manufacturing

A technology of luminophores and antibiotics, which is applied in the field of preparation of AIE luminophores and magnets, can solve the problems of fluorescence quenching, chromophore aggregation, and the reduction of bacterial killing efficiency, and achieve the effect of rapid screening and simplified imaging process

Active Publication Date: 2017-08-01
THE HONG KONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most materials are coplanar or extremely hydrophobic in nature, strong π-π or hydrophobic interactions can occur, which will lead to chromophore aggregation, reduced bacterial killing efficiency, and fluorescence quenching effects

Method used

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  • Aie luminogens for bacteria imaging, killing, photodynamic therapy and antibiotics screening, and their methods of manufacturing
  • Aie luminogens for bacteria imaging, killing, photodynamic therapy and antibiotics screening, and their methods of manufacturing
  • Aie luminogens for bacteria imaging, killing, photodynamic therapy and antibiotics screening, and their methods of manufacturing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0202] The following examples are intended to illustrate the subject-matter of the present application without restricting it.

[0203] Material:

[0204] LB agar, LB medium, anhydrous potassium hydrogen phosphate and sodium phosphate were all purchased from USB Co., Ltd., while the green singlet oxygen sensor (SOSG) was purchased from Invitrogen. Zinc powder, titanium tetrachloride, 4-hydroxybenzophenone, 4-bromobenzophenone, 1-bromoundecane, piperidine, propidium iodide, potassium carbonate, 4-formylphenylboronic acid and Tetrakis(triphenylphosphine)palladium was purchased from Sigma-Aldrich and used as received. THF was directly purified by distillation in sodium benzophenone ketyl before use. 1-(3-Trimethylammoniumpropyl)-4 was synthesized according to the method of Yan et al. -methylpyridinium dibromide. Other reagents used such as dimethylsulfoxide, potassium chloride and sodium chloride were purchased from Sigma-Aldrich.

[0205] Characterization:

[0206]Use CDCl...

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Abstract

The invention relates to synthesis of fluorescent molecules with aggregation-induced emission (AIE) characteristics and application as fluorescent probes for cellular imaging, killing, and antibiotic screening, wherein the cells are bacteria or mammalian cells. The fluorescent molecules generate reactive oxygen species (ROS) upon light irradiation. The invention also relates to a probe comprising fluorescent molecules exhibiting AIE phenomenon, wherein the probe is used for bacterial and biological study. The invention further relates to a method of imaging and quantifying bacterial, a method of killing cells, a method of high throughput antibiotics screening and determining bacteria resistance, a method of photodynamic therapy, and a method of determining critical micelle concentration.

Description

[0001] related application [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 123,596, filed November 21, 2014, the applicant of which is the inventor of this patent application, and is hereby incorporated by reference in its entirety to Reference. technical field [0003] This application relates to the research and development of luminophores with aggregation-induced emission (aggregation-induced emission, AIE) properties, and the application of these materials in bacteria, mammalian cells and related biological research. In particular, the present application relates to the preparation of AIE luminophores and magnetites useful as fluorescent probes for bacterial quantification, imaging, killing, antibiotic screening, and photodynamic therapy (PDT). Background technique [0004] With the improvement of human living standards, hygiene and food safety issues closely related to human health have received widespread attention from scienti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07C43/215C07C217/64C07C211/27C07F7/10C12Q1/00G01N21/64A61K31/00A61P35/00
CPCA61P35/00C07C211/31C07C2603/18C09B23/086C09B23/105C09B23/141C09B23/145C09B23/148C09B57/00C09K11/06C09K2211/1007C09K2211/1011C07C43/215C07F7/10G01N33/533G01N33/582G01N21/64
Inventor 唐本忠赵恩贵
Owner THE HONG KONG UNIV OF SCI & TECH
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