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Tranquilizing composition containing as active ingredient exopolysaccharide produced by means of ceriporia lacerata

A technology for tearing Cerobacteria and exopolysaccharides, which is applied in the functions of food ingredients, microorganisms, food ingredients, etc., can solve problems such as insufficient research on industrialization

Active Publication Date: 2017-08-01
福健生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fact is that since the first report of cereus tearing in the academic circle in 2002, there are still insufficient researches on its industrialization

Method used

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  • Tranquilizing composition containing as active ingredient exopolysaccharide produced by means of ceriporia lacerata
  • Tranquilizing composition containing as active ingredient exopolysaccharide produced by means of ceriporia lacerata
  • Tranquilizing composition containing as active ingredient exopolysaccharide produced by means of ceriporia lacerata

Examples

Experimental program
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manufacture example 1

[0051] Production Example 1. Production of Ceruleus cerevisiae culture solution, its dry powder, extract, and exopolysaccharide (hereinafter referred to as "EPS")

[0052] 1.1 Manufacture of the culture solution of cereus torn fungus

[0053] The mucormyces isolated from living oak trees collected from Sangju, Gyeongsangbuk-do were subcultured to grow Mucorales and stored frozen at -80°C. The preserved strains were stored in PDA (Potato dextrose agar ) medium (87 plastic medium, medium (Difco), Becton, Dickinson and Company (Becton, Dickinson and Company)) subcultured 2 to 3 times, and the strain (hereinafter referred to as "PDA culture strain") was preserved Use in a refrigerator at 4°C. And PDB (Potato dextrose broth) medium (Difco, Becton Dickins on and Company) 600ml was composed behind the Erlenmeyer flask, where a PDA culture strain was placed and shaken at 25°C for 8 days to obtain the PDB culture strain.

[0054] Afterwards, the liquid culture medium was fermented...

Embodiment 1

[0061] The characteristic evaluation of embodiment 1.EPS

[0062] 1.1 Molecules of EPS by Gel Permeation Chromatography (GPC) Quantitative determination

[0063] The EPS produced in the above Production Example 1 was dissolved in 0.1M Na 2 SO 4 / 0.05MNaN 3 (adjust the pH to 4 with glacial acetic acid) solution, centrifuge at 4,000 rpm for 0.5 hours, filter only the supernatant through a 0.45 μm syringe filter, and use GPC for analysis.

[0064] Specifically, for the GPC analysis conditions, the refractive index is used by the detector, for the GPC column, OHpak SB 805 HQ (Shodex, Japan) is used, for the mobile phase, 0.1M Na 2 SO 4 / 0.05M NaN 3 (The pH was adjusted to 4 with glacial acetic acid), and the flow rate of the mobile phase flowed in at 1.0 ml / min. The standard curve utilizes dextran (dextran) (American Polymer Corporation, U.S.) with different molecular weights (130kDa, 400kDa, 770kDa, or 1200kDa) respectively, and utilizes a refractive index (refractive ...

Embodiment 2

[0080] Nervous calming effect verification of embodiment 2.EPS

[0081] In order to investigate the nerve-sedating effect of EPS isolated from the mycelia culture solution of R. cerevisiae, hippocampal single cells isolated from embryonic hippocampal nerve tissues of white mice were treated with the EPS produced in the above Production Example 1. , to measure changes in intracellular calcium concentration. Specifically, the hippocampal nerve tissue was isolated from embryos of 18-19 days pregnant white mice under a dissecting microscope, added with 0.25% trypsin, and allowed to react at 37° C. for 10 minutes. Next, the tissue was washed several times with HBSS (Hanks'Balanced Salt Solution) to remove trypsin, and the hippocampal cells were separated into single cells with a 1 ml pipet. After the isolated hippocampal single cells were treated with the EPS produced in Production Example 1 at a concentration of 100 ug / mL, it was described in the literature (Dunlap K. et al., Tre...

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Abstract

The present invention relates to a tranquilizing composition containing as an active ingredient: exopolysaccharide produced by means of Ceriporia lacerata; a Ceriporia lacerata mycelium culture medium comprising the exopolysaccharide; dry powder of the mycelium culture medium; or an extract of the mycelium culture medium. The composition can be used as a tranquilizer for preventing or treating depression or anxiety or as a functional health food having tranquilizing effect.

Description

technical field [0001] The present invention relates to a composition for calming nerves, which contains the following active ingredients: exopolysaccharide produced by Ceriporia lacerata or lacerations containing exopolysaccharide The mycelium culture fluid, the dry powder or the extract of the mycelia culture fluid. Background technique [0002] The recent trend is that there is a sharp increase in patients with depression or anxiety due to various reasons such as economic problems such as economic growth rate, divorce and children's problems. Anxiety is defined as a largely unpleasant and unconscious feeling of anxiety, with associated physical symptoms (palpitations, sticky sweat, etc.) and behavioral symptoms (anaphylaxis, wandering, etc.) (Robert F .Scmidt, Human physiology, pp.366; Min Sun ggil, Latest Psychiatry, pp.238-40; Argyropoulos S.V. et al., Pharmacol.Ther.88, pp.213-27, 2000). When anxious, the whole brain enters an arousal state, and as a result, periphe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/10A23L31/00A23L33/17A61K36/06
CPCA23V2002/00A61K36/06A23V2200/31A23L31/00A23L33/10A23L33/17Y10S435/853
Inventor 金润秀
Owner 福健生物制药有限公司
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