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Sorghum aphid life table experimental method

An experimental method, the technology of sorghum aphid, is applied in the field of insect life table experiments, which can solve the problems of blocked nutrient transformation, insufficient water, and low survival rate of sorghum aphid, and achieve the effect of convenient operation, simple method and easy implementation

Inactive Publication Date: 2017-08-01
ANHUI SCI & TECH UNIV
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Problems solved by technology

[0002] Life table is an experimental method for studying the growth and development and biological characteristics of insects. Sorghum aphid is the main pest that harms sorghum, sudangrass and sorghum grass. At present, the life table experiment method of sorghum aphid uses detached leaves to test in a petri dish. The disadvantages of using detached leaves are: the water is insufficient after the leaves are separated from the plant, and the transformation of nutrients is blocked. Compared with fresh plants, there is a certain gap in nutrients and water, and the living conditions of sorghum aphids on sorghum plants cannot be accurately simulated.
Therefore, pre-tests in the laboratory found that the survival rate of sorghum aphids on detached sorghum leaves and stems is low

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  • Sorghum aphid life table experimental method

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Embodiment Construction

[0009] Below in conjunction with embodiment further illustrate the present invention.

[0010] In this embodiment, the selected vessel is made by remodeling a commercially available mineral water bottle. The specific method is: cut off the conical part of the mineral water bottle near the mouth of the bottle, and keep the cylindrical part.

[0011] After completing the above selection, this embodiment can be carried out according to the following steps:

[0012] (1), see figure 1 The inner chamber of the vessel 1 has a depth of 15-20 cm, a window 1a is opened on the top of the vessel 1, and more than two vent holes 1b are opened at the bottom of the vessel 1. In this embodiment, the bottom of the vessel 1 is opened. There are five ventilation holes 1b, and the window 1a opened on the top of the container 1 has a height of 10cm and a width of 2cm. Add soil containing nutrient soil in the container, and the height of the soil is 1 / 4-1 / 3 of the depth of the container cavity. ; ...

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Abstract

A sorghum aphid life table experimental method mainly comprises the steps of adding soil into a vessel; cultivating sorghum seedlings in the soil; covering the surface of the soil with white paper; inoculating female sorghum aphids to the sorghum seedlings, and removing the female aphids with young aphids are reserved; subjecting the single seedling and seedling aphid to life table data recording, recording survival condition, molting time and frequency of the aphids per observation of the young aphids, removing the skin of the young aphids fallen on the white paper, recording aphid survival condition and aphid production quantity per observation of adult aphids, and removing and discarding the young aphids from the adult aphids until the aphids are dead. In the method provided herein, sorghum seedlings can provide sufficient moisture and nutrition for sorghum aphids in the experiment, and conditions are provided for accurately simulating the free living state of sorghum aphids on field sorghum plants; the skin molted by the aphids falls onto the white paper for the purpose of accurate observation and recording; the vessel can ensure that the molted skin of the sorghum aphids never drifts away; observation is facilitated and visualized through a window.

Description

technical field [0001] The invention relates to an experimental method for the life table of insects, in particular to an experimental method for the life table of sorghum aphids. Background technique [0002] Life table is an experimental method for studying the growth and development and biological characteristics of insects. Sorghum aphid is the main pest that harms sorghum, sudangrass and sorghum grass. At present, the life table experiment method of sorghum aphid uses detached leaves to test in a petri dish. The disadvantages of using detached leaves are: the water is insufficient after the leaves are separated from the plant, and the transformation of nutrients is blocked. Compared with fresh plants, there is a certain gap in nutrients and water, and the living conditions of sorghum aphids on sorghum plants cannot be accurately simulated. Therefore, pre-tests in the laboratory found that the survival rate of sorghum aphids on detached sorghum leaves and stems is low. ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 杜军利武德功詹秋文黄保宏何克勤
Owner ANHUI SCI & TECH UNIV
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