Second harmonic imaging method and second harmonic imaging device for single suspended particles

A second harmonic, imaging method technology, applied in the field of optical imaging, can solve problems such as insufficient resolution, difficulty in PM2.5 particles, inability to distinguish condensed water and condensation nuclei, and achieve high capture efficiency and low cost Effect

Inactive Publication Date: 2017-07-28
NORTHWEST UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, because the resolution of traditional optical microscopy imaging is not high enough to distinguish condensation water and condensation nuclei, and PM2.5 particles are usually in a floating state, it is usually very difficult to directly observe individual PM2.5 particles

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  • Second harmonic imaging method and second harmonic imaging device for single suspended particles

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Embodiment 1

[0030] This embodiment provides a second harmonic imaging method for a single suspended particle, comprising the following steps:

[0031] Step 1: A Bessel beam is incident on the sample chamber 9 containing a single fine particle along an optical path, and a Bessel beam standing wave field is generated in the sample chamber by means of interference; the diameter of the fine particle is smaller than 10 μm; the standing wave field of the Bebessel beam obtained can capture the three-dimensional information of fine particles;

[0032] Step 2: Focus a beam of femtosecond laser on the sample chamber, so that the femtosecond laser and the Bessel beam are collinear, and the fundamental frequency light and the second harmonic signal emitted from the sample chamber are separated and imaged separately; the femtosecond laser and The captured fine particles interact to generate a second harmonic signal;

[0033] Step 3: Adjust the size of the bright spot in the center of the Bessel beam ...

Embodiment 2

[0036] For the second harmonic imaging device for single suspended particles provided by the present invention, see figure 1 , including a femtosecond laser 1, a continuous laser 2, a laser beam expander 3, a Bessel beam generating device 4, a first mirror 5, a second mirror 6, a convex lens 7, a first microscope objective lens 8, and a sample chamber 9 , the second microscope objective lens 10, the third mirror 11, the first focusing lens 12, the first imaging device 13, the optical filter 14, the second focusing lens 15, the second imaging device 16, the rear quartz window 17, the front quartz window 18.

[0037] Among them, the femtosecond laser 1 is a laser with an adjustable wavelength in the range of 750-990nm; the central wavelength of the continuous laser 2 is 532nm; the sample chamber 9 contains fine particles (diameter less than 10 μm) that can freely float; the laser beam expander 3 The beam expansion factor is adjustable within 2-10 times; the back focus of the co...

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Abstract

The invention discloses a second harmonic imaging method and a second harmonic imaging device for single suspended particles. According to the method, Bessel laser is adopted for effectively capturing the suspended particles, and remote noncontact and noninvasive capture and high capture efficiency are realized; imaging is realized by second harmonic signals generated on the particles by femtosecond laser, polarization resolution, spectral resolution and three-dimensional imaging are realized, and imaging and observation of fine particles are realized. The device for implementing the second harmonic imaging method has advantages that due to adoption of common optical devices, low cost is realized while imaging of the second harmonic signals can be well realized, and suitableness for popularization is achieved.

Description

technical field [0001] The invention belongs to the field of optical imaging, and in particular relates to a second harmonic imaging method and device suitable for single suspended particles based on laser capture. Background technique [0002] Particulate pollutants floating in the atmosphere are not only one of the important factors causing environmental problems such as reduced atmospheric visibility and photochemical smog, but more seriously, fine particulate matter (PM2.5) with a diameter of less than 2.5 μm can enter the human body through the respiratory system and penetrate deeply Lungs and cardiovascular system, causing damage to the respiratory system and cardiovascular system. Studies have established that PM2.5 pollutants directly contribute to asthma, lung cancer, cardiovascular disease, birth defects and premature death. At present, PM2.5 pollution has become the most prominent atmospheric environmental problem in our country, seriously threatening the life an...

Claims

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Application Information

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IPC IPC(8): G01N21/63G01N21/01
CPCG01N21/01G01N21/63
Inventor 程雪梅陈浩伟张倩任兆玉白晋涛
Owner NORTHWEST UNIV
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