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Liquor-fermentation production method of neutral protease

A neutral protease and production method technology, which is applied in the field of microbial fermentation production, can solve the problems of high solid content and low unit enzyme activity, and achieve the effects of shortening the lag period, good heat resistance, and reducing the risk of bacterial contamination

Inactive Publication Date: 2017-07-28
WUHAN SUNHY BIOLOGICAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the problem that there are many solids and low unit enzyme activity in the traditional medium of neutral protease

Method used

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  • Liquor-fermentation production method of neutral protease
  • Liquor-fermentation production method of neutral protease
  • Liquor-fermentation production method of neutral protease

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Embodiment Construction

[0024] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] Embodiment provides a kind of supernatant liquid fermentation production method of neutral protease, specifically comprises the following steps:

[0026] (1) Activation of refrigerated bacterial strains in glycerol tubes: Take refrigerated bacterial strains in glycerin tubes, pick up the bacterial strains with an inoculation needle and inoculate them into a test tube filled with sterile water, shake for 10-20 minutes, and then take 0.2-1ml of bacterial liquid with a sterile pipette Add it to the slant medium, place it in a co...

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Abstract

The invention provides a liquor-fermentation production method of neutral protease. The liquor-fermentation production method includes 1), culture activation, 2), seed cultivation, 3), liquor fermentation, and 4), extracting and refining of the neutral protease. The liquor-fermentation production method of the neutral protease has the advantages that through optimization of culture media and complete adoption of soluble culture media, the problems of large quantity of solid matters in existing culture media, high viscosity of the existing culture media, difficulty in after-treatment and high cost are solved; products with high enzyme activity are obtained through process optimization.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation production, and in particular relates to a clear liquid fermentation production method of neutral protease. Background technique [0002] Protease refers to a class of protease with the optimum pH between 6.0 and 7.5, which can catalyze the hydrolysis of protein peptide bonds. Neutral protease was the first to be discovered and widely used in production. Due to its properties and advantages such as fast catalytic reaction speed, no industrial pollution, and wide adaptability to catalytic reaction conditions, it is widely used in food, pharmaceuticals, cosmetics, detergents, etc. , silk spinning, fur softening and other industries. [0003] The production of neutral protease can be extracted from animal and plant tissues. Plants as the source of protease will be affected by factors such as climatic conditions and the size of available land area, while animals are used as the source ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/54C12R1/125
CPCC12N9/54
Inventor 熊晓燕谌颉张立张成杰
Owner WUHAN SUNHY BIOLOGICAL
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