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Anti-inflammatory effect of a yeast oxidative stress metabolite and its application

A technology for oxidative stress, anti-inflammatory effects, applied in the field of microbiology

Active Publication Date: 2020-08-11
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-inflammatory activity of LYCD has not been reported yet.

Method used

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  • Anti-inflammatory effect of a yeast oxidative stress metabolite and its application
  • Anti-inflammatory effect of a yeast oxidative stress metabolite and its application
  • Anti-inflammatory effect of a yeast oxidative stress metabolite and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Yeast oxidative stress metabolites provided by the present invention are obtained by adding 0.02-0.4mM H 2 o 2 After treatment for 10-60 minutes, centrifuge at 10000r / min for 5min, take the precipitate, wash once with PBS, centrifuge at 10000r / min for 5min, take the precipitate, replace with new YEPD medium, and continue culturing for 1-6h (conditional temperature is 28°C-30°C , rotate at 120-200r / min, centrifuge (10000r / min, 5min), wash twice with PBS, take the precipitated part, add normal saline (according to 1g (wet weight) / 1mL), freeze and thaw repeatedly 5 times (liquid nitrogen method ), centrifuge 3 times (10000r / min, 10min), take the supernatant and store it at -20°C.

[0045] The prepared yeast oxidative stress metabolite samples were taken to detect their antioxidant capacity. The results showed that the GSH content was 0.3881 μg / mL; the SOD activity was 12.6020U / mgprot; and the CAT activity was 2.7371U / mgprot. It shows that the obtained active yeast cell d...

Embodiment 2

[0047]The anti-inflammatory activity of LYCD was evaluated by xylene-induced mouse ear swelling model;

[0048] (1) Randomly divide some Kunming mice into blank group, acetone group, normal saline group, TPA group, and experimental group (LYCD concentration is 1.0mg / mL, 2.0mg / mL, 4mg / mL, 8mg / mL, 16mg / mL), NAC (N-acetylcysteine, 10mg / mL) group, 3 mice in each group, weighed, repeated 3 times;

[0049] (2) Except for the blank group and TPA group, 10 μL of LYCD was applied to each group, once every hour, twice;

[0050] (3) After 2 hours, except that the blank group was not treated, 8ul of acetone was evenly applied on both sides of the ears of the mice in the acetone group, and 10μL of normal saline was evenly applied on both sides of the ears of the mice in the normal saline group. Apply 8 μL of TPA with a concentration of 0.015mM in the same way;

[0051] (4) After an interval of 2 hours, repeat the operation in step 2, and then repeat the operation in step 2 again after an...

Embodiment 3

[0058] 1. Drawing materials

[0059] Take fresh mouse tissue and fix it in 4% paraformin for more than 24h. Take out the tissue and organize the target site. After completion, put the tissue and corresponding labels into the dehydration box. Place the trimmed tissue and the corresponding label in the dehydration box.

[0060] 2. Dehydration

[0061] Put the above dehydration box into the hanging basket and place it in the dehydrator to dehydrate with gradient alcohol sequentially. 75% alcohol 4h-85% alcohol 2h-95% alcohol 2h-90% alcohol 1h-absolute ethanol I30min-absolute ethanol II 30min-alcohol benzene 5-10min-xylene I5~10min-xylene II 5~10min - Wax I 1h - Wax II 1h - Wax III 1h.

[0062] 3. Embedding

[0063] Put the above wax-soaked tissue samples into the embedding machine for embedding. Put the melted wax into the embedding frame first, take out the tissue from the dehydration box before the wax solidifies, put it into the embedding frame according to the embedding...

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Abstract

The invention relates to the technical field of microorganisms, in particular relates to an anti-inflammatory effect of oxidative stress yeast metabolite and application of the oxidative stress yeast metabolite in preparation of anti-inflammatory products. The invention provides a preparation method of the oxidative stress yeast metabolite; an experiment shows that the yeast metabolite can inhibit expression of COX-2 and activation of an NF-kappaB signal channel and can play effects of inhibiting inflammatory cell infiltration, inhibiting increment of nuclear-cytoplasmic ratio of cells, and inhibiting epidermal keratinization and / or reducing swelling degree so as to prove that the yeast metabolite has an anti-inflammatory effect, can be applied to related fields of drugs, cosmetics, health care products, food and the like, and can be made into dosage forms such as tablets, capsules, cream and liquid.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of yeast metabolites in the preparation of anti-inflammatory products. Background technique [0002] As an important pathological process, inflammation is very common in the human body. It is the defense response of living tissues in the vascular system of the body to damage factors, and is the tissue damage caused by damage factors. Therefore, most diseases are accompanied by the mediation and occurrence of inflammation. [0003] When yeast cells are under stress, such as ultraviolet radiation, high and low temperature, oxidative stress, etc., they will initiate a variety of stress mechanisms to produce a variety of active substances to resist adverse environmental stimuli. These active substances are yeast oxidative stress metabolites , also known as active yeast derivatives (Live Yeast Cell Derivative, referred to as LYCD). The original LYCD was extrac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12N1/16C12N1/06A61K36/064A61P29/00
CPCA61K36/064C12N1/063C12N1/16C12N1/18
Inventor 杜志云彭奕梁邱闯张焜郑希方岩雄周渭汤亚东张蓝月黎鹏辉
Owner GUANGDONG UNIV OF TECH
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