A nanotube-based nanopore detection system and its preparation method and application

A detection system and nanotube technology are applied to a nanopore detection system for detecting long-chain polymers and the field of preparation thereof, which can solve the problems of poor mechanical stability of two-dimensional material films, reduced signal-to-noise ratio, and low spatial resolution, etc. The effect of good mechanical stability, improved resolution and sensitivity

Active Publication Date: 2020-09-29
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI +1
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Problems solved by technology

First, the mechanical stability of 2D material films is poor
The two-dimensional material film with sub-nanometer thickness is prone to mechanical vibration in the electrolyte solution, and the vibration noise generated will make the weak signal generated by the ionic current submerged in the noise and cannot be recognized; second, two-dimensional materials such as graphene are transferred to its support. Defects will be generated in the substrate process, and the leakage current generated by the defects is also considered to be one of the reasons for the low signal-to-noise ratio of the current signal detected by the nanopores of two-dimensional materials.
Third, compared with traditional silicon-based nanopores, although the atomic-level channel length of two-dimensional material nanopores can improve the spatial resolution of current signals, it also allows DNA molecules to maintain a greater degree of freedom when passing through the pores. This leads to an unstable molecular configuration and reduces the signal-to-noise ratio
[0004] Most of the existing nanotube biosensors based on the nanopore detection principle integrate nanotubes into silicon-based nanopores (CN103796947B) or use radial slices of nanotubes to form nanopores (CN 1994864B), but the nanotubes formed by these methods hole, there are still problems of low spatial resolution and low signal-to-noise ratio

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  • A nanotube-based nanopore detection system and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This embodiment provides a nanopore detection system based on single-walled carbon nanotubes. The specific implementation is as follows:

[0045] It is 1-10nm to grow diameter on the substrate 5 surface that has silicon dioxide thin film, and length is 500-1000 μm nanotube 14, on above-mentioned substrate 5 with nanotube 14, spin-coat negative photoresist, use light The engraving processing method is to process a negative photoresist microchannel structure 6 with a thickness of 20 μm and a width of 7 μm on the selected nanotube 14 with a diameter of 2 nm and a length of 500 μm to form the first chamber 11 and the second chamber. Chamber 12 and third chamber 13.

[0046]There is no communication between the first chamber 11 and the second chamber 12 , and between the second chamber 12 and the third chamber 13 , and the communication between the first chamber 11 and the third chamber 13 is only through the nanotube 14 . The two ends of the nanotube 14 are treated with a...

Embodiment 2

[0051] This embodiment provides a nanopore detection system based on boron nitride nanotubes. The specific implementation manner is as follows:

[0052] Dispersed on the surface of the substrate 5 with a diameter of 300-500nm and a length of 10-90 μm nanotubes 14, spin-coat positive photoresist on the above-mentioned substrate with nanotubes. A positive photoresist microchannel structure 6 with a thickness of 15 μm and a width of 8 μm is processed on the nanotube 14 with a thickness of 400 nm and a length of 20 μm to form a first chamber 11 , a second chamber 12 and a third chamber 13 .

[0053] There is no communication between the first chamber 11 and the second chamber 12, the second chamber 12 and the third chamber 13, and only the selected nanotubes 14 communicate between the first chamber 11 and the third chamber 13 . The two ends of the nanotube 14 are treated with a focused ion beam to ensure that the two ends of the nanotube 14 are not blocked. The part of the nano...

Embodiment 3

[0058] This embodiment provides a nanopore detection system based on multi-walled carbon nanotubes. The specific implementation manner is as follows:

[0059] Growth diameter is 10-90nm on the surface of substrate 5, and length is 100-500 μm nanotube, on above-mentioned substrate 5 with nanotube, spin coating negative photoresist, with the method for photolithographic processing, in selected A negative photoresist microchannel structure 6 with a thickness of 25 μm and a width of 5 μm is processed on the nanotube 14 to form a first chamber 11 , a second chamber 12 and a third chamber 13 .

[0060] There is no communication between the first chamber 11 and the second chamber 12 , and between the second chamber 12 and the third chamber 13 , and the communication between the first chamber 11 and the third chamber 13 is only through the nanotube 14 . The two ends of the nanotube 14 are treated with a focused ion beam to ensure that the two ends of the nanotube 14 are not blocked. ...

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Abstract

The invention discloses a nano-pore detection system based on a nano-tube and a preparation method and application thereof. The system comprises a nano-pore structure based on the nano-tube, a micro-channel structure, a cover plate for sealing the micro-channel structure, and a current detection system for detecting a to-be-detected object. The nano-pore structure based on the nano-tube comprises a substrate and the nano-tube. The nano-tube is dispersed or grows on the substrate, openings are formed in the left end and the right end of the nano-tube, and a nano-pore is formed in the side wall. The micro-channel structure is machined on the substrate and provided with three independent electrolyte solution cavities, the nano-tube penetrates through the three cavities, and the left opening, the nano-pore and the right opening of the nano-tube are communicated with the first cavity, the second cavity and the third cavity respectively. One end of the current detection system is arranged in the second cavity, and the other end of the current detection system is arranged in the first cavity or the third cavity. The resolution and sensitivity of a bio-sensor can be greatly improved, and the concept of single molecule DNA direct sequencing is expected to be achieved.

Description

technical field [0001] The invention relates to the technical field of nanopore detection systems, in particular to a nanopore detection system based on a nanotube structure that can be used to detect long-chain polymers (DNA, RNA or polypeptide) and a preparation method thereof. Background technique [0002] Nanopore technology is a powerful tool for the analysis of biomolecules, and it has been specifically applied to the following three fields: First, biophysical research at the single-molecule level, such as the folding / unfolding of DNA molecules, the melting process of double-stranded DNA, Single-molecule DNA-protein interaction and force spectrum measurement of biomolecules, etc.; second, generalized "early diagnosis" at the single-molecule level. Such as identification of important attributes of single biomolecules, identification of specific short fragments, etc.; third, direct sequencing of single-molecule DNA. [0003] As the first nanopore to be used in DNA seque...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 王赟姣王德强刘梦婉邓云生袁家虎
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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