Method for determining formation time of corn pollen tube pathways

Abstract

The invention provides a method for determining formation time of corn pollen tube pathways. The method comprises steps as follows: bagging, pollination, cutting up of bracteal leaves, removal of filaments and recovery of the bracteal leaves to original state, specifically, the filaments are removed every 1-3 h, temperature and humidity are recorded in real time, female spikes are taken back during harvesting, the number of mature grains is counted, and the formation time of the corn pollen tube pathways is determined according to the time of filament removal and the average number of mature grains. The method is implemented in the fields and is closest to the actual planting condition, and a result is high in reliability; steps of the method are simple and are easily mastered and operated by technical personnel in the field; the method is high in feasibility and good in repeatability and can be used for different materials; besides, the method has lower requirements for operation conditions and is low in cost.

Description

technical field [0001] The invention belongs to the technical field of agriculture, in particular to a method for determining the formation time of corn pollen tube channels. Background technique [0002] Since the method of pollen tube passage was founded by Chinese scientist Zhou Guangyu in 1983, it has been widely used in maize transgenic breeding because it does not require tissue culture and plant regeneration, and has the characteristics of simple technology, easy to master and low equipment requirements. The principle of this method is that the pollen tube channel formed by pollination germination can transfer germ cell DNA and foreign DNA into egg cells and finally transform into zygote or early embryo cells. It can be seen that only when the pollen tube channel is completely opened, can the carried sperm be transported to the egg cell for fertilization and final fruiting. Therefore, the key to using the pollen tube channel method is to determine the time period for ...

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Problems solved by technology

[0004] At present, in the research of maize transgenic breeding using the pollen tube channel method, the transformation time is concentrated between 8-30 hours, the time span is relatively large, and the meteorological conditions between materials and years are also different, which results in the transformation efficiency of the pollen tube channel method. Not high, poor repeatability of results, unfavorable for reference between different materials, etc.

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