A kind of lung cancer monitoring kit and using method thereof

Abstract

A lung cancer monitoring kit and an application method thereof. The monitoring kit comprises: a reagent A prepared by adding 5% of SDS into an ammonium bicarbonate solution having a concentration of 10 mM; a reagent B prepared by adding not less than 2.2 unit / μL of an exoglycosidase into NP40 having a concentration of 3.3%; a reagent C comprising a mixed solution of equal volumes of 20 mM of APTS in 1.2 M of a citric acid and 1 M of an organic reducing agent in DMSO; and a reagent D comprising 0.25 μL NH4AC having a concentration of 100 mM and pH 5, 0.2 μL of a neuraminidase, and 2.55 μL hydrogen peroxide. The kit is used to evaluate a lung cancer patient on the basis of a diagnostic indicator of a fingerprinting pattern generated by detecting oligosaccharide chains in a blood glycoprotein, so as to diagnose a tumor stage of a lung cancer, implementing regular and non-invasive detection for a lung cancer patient, and monitoring the progress of the disease in real time.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a lung cancer monitoring kit and a use method thereof. Background technique [0002] Worldwide, lung cancer is the leading cause of cancer death in both men and women. In 2005, it was estimated that there were about 500,000 new cases of lung cancer in China (about 330,000 cases in men and about 170,000 cases in women). There are two main reasons for the high mortality rate of lung cancer: one is the lack of effective early diagnosis methods for lung cancer, and more than 70% of the patients are diagnosed at an advanced stage; the other is that advanced lung cancer (especially non-small cell lung cancer, NSCLC) is sensitive to chemotherapy The overall effective rate of various existing chemotherapy regimens for NSCLC is only about 30%. [0003] Lung cancer can be divided into two types according to anatomical classification: central lung cancer and peripheral lun...

AI Technical Summary

Problems solved by technology

There are two main reasons for the high mortality rate of lung cancer: one is the lack of effective early diagnosis methods for lung cancer, and more than 70% of the patients are diagnosed at an advanced stage; the other is that advanced lung cancer (especially non-small cell lung cancer, NSCLC) is sensitive to chemotherapy The overall effective rate of various existing chemotherapy regimens for NSCLC is only about 30%.

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