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Prostate cancer detection reagent and application thereof in prostate cancer detection

A technology for prostate cancer and detection reagents, applied in the field of biomedicine, can solve the problems of small injection volume, expensive mass spectrometer and high sensitivity, and achieve the effect of obvious accuracy

Pending Publication Date: 2022-02-11
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Description
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  • Application Information

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Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

Method used

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  • Prostate cancer detection reagent and application thereof in prostate cancer detection
  • Prostate cancer detection reagent and application thereof in prostate cancer detection
  • Prostate cancer detection reagent and application thereof in prostate cancer detection

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Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1 detects prostate cancer

[0054] Materials and methods used:

[0055] 1. Test samples: serum from prostate cancer patients and normal controls.

[0056] 2. Experimental equipment: capillary electrophoresis analyzer, PCR, centrifuge.

[0057] 3. Reagent preparation:

[0058] Reagent A: prepared by adding SDS with a mass concentration of 0.5 to 5% in ammonium bicarbonate solution with a concentration of 10 mM;

[0059] Reagent B: It is prepared by mixing 0.01~10U / 10μL glucosamidase and 0.01~10U / 10μL sialidase, and the pH value of the mixed solution is 4~9;

[0060] Reagent C: Prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO, the concentration is 0.01mM~1M;

[0061] Reagent D: stop solution.

[0062] 4. Glycosequencing detection steps:

[0063] Step 1 Preparation of oligosaccharide chains

[0064] Add 4 μL of reagent A to 2 μL of inactivated serum samples to denature, after cooling down to room temperature, add 4 μL of reagent B, and ...

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Abstract

The invention provides a prostate cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. The invention provides a method for establishing a model of a serum glycome map of prostate cancer to detect the prostate cancer by measuring the glycome map in the serum through a detection reagent and quantifying peak values for statistical analysis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for detecting prostate cancer, in particular to a method for detecting prostate cancer based on a serum glycoprotein oligosaccharide chain detection (G-Test) map. Background technique [0002] Prostate cancer is an epithelial malignant tumor that occurs in the prostate gland and is the most common malignant tumor in the male genitourinary system. Prostate cancer is a cancer that progresses very slowly. It is not easy to detect in the early stage of the disease. The clinical manifestations of patients are urinary tract symptoms such as dysuria, low back pain, urgency, frequent urination, and painful urination. The main treatment is radical prostatectomy, surgery or drug castration. And other treatment, early prostate cancer can be cured, advanced conservative treatment. [0003] The etiology of prostate cancer is related to heredity, environment, food, and age. If there...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/924G01N2333/98G01N2800/7028
Inventor 陈翠英王蕾谈宗男
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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