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Detection reagent for pancreatic cancer and application of reagent in pancreatic cancer detection

A detection reagent and a technology for pancreatic cancer, applied in the field of biomedicine, can solve the problems of traumatic pathological examination, poor patient dependence, low specificity and accuracy of blood testing and imaging detection, and achieve high repeatability and stability Good, high-sensitivity effect

Pending Publication Date: 2019-04-26
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems that in the existing detection of pancreatic cancer, the specificity and accuracy of blood detection and imaging detection are not high, the pathological examination is invasive, sampling error is poor, and the patient dependence is poor, the present invention provides a pancreatic cancer N-oligo Sugar chain detection method

Method used

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  • Detection reagent for pancreatic cancer and application of reagent in pancreatic cancer detection
  • Detection reagent for pancreatic cancer and application of reagent in pancreatic cancer detection
  • Detection reagent for pancreatic cancer and application of reagent in pancreatic cancer detection

Examples

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example 1

[0033] (1) Experimental equipment:

[0034] ABI3500dx analyzer (Applied Biosystems), PCR, centrifuge

[0035] (2) Reagent preparation:

[0036] PDACS1: NH at 10 mM 4 HCO 3 , formulated as NH containing no more than 5% SDS and pH 7.5~8.0 4 HCO 3 solution.

[0037] PDACS 2: Mix PNGaseF at a concentration of 2~5U / µL, sialidase at a concentration of 1~2mU / µL, and hydrogen peroxide at a volume ratio of 1:1:13.

[0038] PDACS 3: mix equal volumes of 20mM APTS and 1M NaCNBH 3 .

[0039] PDACS 4: Buffer.

[0040] (3) N-oligosaccharide chain detection

[0041] a. Preparation of N-oligosaccharide chains: Take 2 µL of serum inactivated at 95°C, add 2 µL of PDACS1, mix and let stand for 5 minutes, add 2 µL of PDACS2 reagent, react at 37-40°C for 3 hours, and then dry.

[0042] b. Labeling of N-oligosaccharide chains: Add 2 µL of reagent PDACS3 to the dried sample, perform fluorescent labeling at no lower than 60°C for 3 hours, then add 200 µL of buffer to terminate the reaction....

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Abstract

The invention provides a detection reagent for the pancreatic cancer. A method comprises PDACS 1, 10 nM of NH4HCO3 is prepared into an NH4HCO3 solution with SDS larger than or equal to 5.0% and pH of7.5-8.0; PDACS 2, 2-5U / muL PNGaseF, 1-2mU / muL neuraminidase and hydrogen peroxide are mixed according to the volume proportion of 1:1:13; PDACS 3, 20 mM of APTS and 1 M of NaCNBH3 with the same volumeare mixed; PDACS 4, a buffer solution is prepared. The invention further provides application of a composite in the detection reagent for the pancreatic cancer. The composite is composed of NGA2F andNG1A2F and is used for detecting the pancreatic cancer through the specific value of NGA2F to NG1A2F. The detection method is high in sensitivity, easy to operate, high in repeatability, good in stability and high in flux and only needs micro samples, and compared with the prior art, the accuracy reaches 86.3% and is obviously superior than that of an existing detection method.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a detection reagent for pancreatic cancer and its application in detection of pancreatic cancer. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat. About 90% of pancreatic cancers are ductal adenocarcinomas originating from the ductal epithelium. The 5-year survival rate is <5%, and it is one of the malignant tumors with the worst prognosis. The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low. The incidence of pancreatic cancer is on the rise rapidly. According to the latest statistics from the National Cancer Center of China, pancreatic cancer ranks 8th in the incidence of male malignant tumors in Chinese cities, and 5th in the mortality rate of malignant tumors in large cities (Beijing, Shanghai)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/574G01N33/57438
Inventor 陈萃英谈宗男朱宏章杨昕高文涛苗毅
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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