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Method for recycling and utilizing glutamic acid fermentation waste bacteria

A technology of glutamic acid and waste bacteria, applied in the field of microorganisms, can solve the problems of difficult removal of impurities, dark color of the hydrolyzate, low purity of impurities, etc., and achieves the effects of improving purity, low cost and improving income.

Inactive Publication Date: 2017-07-14
卢松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of oligopeptides by proteolysis mainly includes enzymatic hydrolysis and acid hydrolysis. Both have their own advantages and disadvantages. Enzymatic hydrolysis is relatively mild, with few impurities and high purity, and relatively high cost; acid hydrolysis is relatively low in cost, but the color of the hydrolyzed solution Deeper, difficult to remove impurities, lower product purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for reclaiming and utilizing glutamic acid to ferment waste thalline comprises the following steps:

[0026] Step 1) Centrifugation: Separating the glutamic acid fermentation broth through a disc separator at a speed of 4000rpm and a centrifugation time of 5min to collect bacterial sediment;

[0027] Step 2) Drying and crushing: air-dry the bacterial cell precipitation naturally, and then pulverize it into bacterial cell powder with a pulverizer;

[0028] Step 3) Salt treatment and ultrasonic treatment: Add the same mass of 5wt% sodium chloride aqueous solution to the bacterial powder, adjust the pH to 2 with sulfuric acid, and use 20kHz ultrasonic treatment for 30 minutes;

[0029] Step 4) Hydrolysis: Put the material obtained in step 3) in the reactor, control the pressure in the reactor to 1MPa, the temperature to 100°C, keep the pressure for 8 minutes, then reduce the pressure to normal pressure, maintain the temperature at 100°C, and continue to add Sul...

Embodiment 2

[0035] The method for reclaiming and utilizing glutamic acid to ferment waste thalline comprises the following steps:

[0036] Step 1) Centrifugation: Separate the glutamic acid fermentation broth through a disc separator at a speed of 5000rpm and a centrifugation time of 3min to collect bacterial precipitates;

[0037] Step 2) Drying and crushing: air-dry the bacterial cell precipitation naturally, and then pulverize it into bacterial cell powder with a pulverizer;

[0038] Step 3) Salt treatment and ultrasonic treatment: Add the same mass of sodium chloride aqueous solution with a concentration of 7wt% to the bacterial powder, and adjust the pH to 2 with sulfuric acid, and use 20kHz ultrasonic treatment for 20 minutes;

[0039] Step 4) Hydrolysis: Put the material obtained in step 3) in the reactor, control the pressure in the reactor to 0.8MPa, the temperature to 100°C, keep the heat for 8 minutes, then reduce the pressure to normal pressure, maintain the temperature at 100...

Embodiment 3

[0045] Taking Corynebacterium glutamicum for fermentation to prepare glutamic acid as an example, the content analysis of each component was detected (measured by dry weight); crude protein 74-76%, nucleic acid 6.5-7%, crude fat 11-12%; Other balance.

[0046] After analysis: Corynebacterium glutamicum contains 17 kinds of amino acids, including 7 kinds of 8 kinds of amino acids necessary for human body, the content of essential amino acids accounts for about 40% of the total amino acid content, and the content of non-essential amino acids accounts for 60% of the total amino acid content. %, the types and contents of amino acids after hydrolysis are relatively rich, and it is an ideal raw material for the production of protein hydrolyzate oligopeptides.

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Abstract

The invention belongs to the technical field of microbiology, and discloses a method for recycling and utilizing glutamic acid fermentation waste bacteria. The method comprises the following steps of (1) centrifuging; (2) stoving and smashing; (3) salt treatment and ultrasonic treatment; (4) hydrolyzation; (5) decolouring and centrifuging; (6) filtering; (7) evaporating and drying; and (8) preparation of fodder. The preparation method is used for preparing oligopeptide products and fodder products, thereby increasing the industrial added value.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for recycling and utilizing glutamic acid fermentation waste bacteria. Background technique [0002] Glutamic acid fermentation bacteria is a single-cell protein. At present, the fermentation waste liquid of MSG factories in my country contains a large amount of glutamic acid bacteria. Many factories discharge it directly, which not only causes serious environmental pollution but also wastes a lot of protein. . Many foreign monosodium glutamate factories recycle glutamic acid bacteria as feed protein. According to reports, among the condiments consumed in Japan and the United States in recent years, the measured consumption of protein hydrolyzate has been equal to or even exceeded the consumption of monosodium glutamate due to its rich amino acid content and high nutritional value. [0003] Fufeng Group is the world's leading enterprise in the production of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12C07K1/14C07K1/34A23K10/16A23K10/37A23K10/26
CPCC07K1/12C07K1/14C07K1/34Y02P60/87
Inventor 许传娟丁兆堂卢松伏广好李晓永徐淑伟王彦妮吴明殷
Owner 卢松
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