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A method for preparing coded microspheres

A technology for coding microspheres and coding materials, which is applied in the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., to achieve the effects of being conducive to wide-scale promotion, simple preparation process and excellent coding performance

Active Publication Date: 2019-06-21
广州广华深启科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, none of the above composite methods can provide a universal solution for different coding materials.

Method used

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  • A method for preparing coded microspheres
  • A method for preparing coded microspheres
  • A method for preparing coded microspheres

Examples

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preparation example Construction

[0028] The present invention provides a method for preparing coded microspheres, in a specific embodiment, comprising the following steps:

[0029] (1) Mix the encoding material with the water-based oligomer, add the corresponding water-based photoinitiator to dissolve completely, and oscillate and stir evenly to form a stable suspension as the water phase;

[0030] (2) mixing and shaking the alkane solvent and emulsifier evenly to form an oil phase;

[0031] (3) drop the water phase in step (1) into the oil phase in the stirring step (2), the volume of the oil phase is 20-30 times the volume of the water phase, drop Continue to stir until the suspension system is uniformly suspended after the addition, and continuously irradiate the suspension system with ultraviolet light;

[0032] (4) After the cured product is washed several times, the microspheres with the target particle size are screened out, and the coded microspheres are obtained after drying.

[0033] In some prefe...

Embodiment 1

[0045]Mix 100 μl of water-soluble 520nm CdSe / ZnS quantum dots with 1 ml of polyethylene glycol diacrylate, add 20 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methyl Propiophenone is completely dissolved, shake and stir evenly to form a stable suspension as the water phase; take 20 ml of hexadecane, add 1 ml of emulsifier cetyl polyethylene glycol, mix and shake evenly to form an oil phase; put the oil phase into Put a magnet in a small beaker, stir magnetically at 600 rpm, drop the water phase into the stirring oil phase with a straw, after the addition is complete, keep stirring for 1 minute until evenly suspended, then turn on the UV lamp (wavelength 355-375nm, energy 200mW) placed above a small beaker, continue to irradiate, and the curing time lasts for 10 minutes; take out the cured suspension and centrifuge it with a mixed washing solution of cyclohexane and ethanol (V / V, 1:1) Wash several times, suspend the remaining solid in ethanol, use a stainless steel mic...

Embodiment 2

[0048] Mix 10 mg of zinc oxide nanoparticles with 1 ml of polyethylene glycol diacrylate, add 20 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone to dissolve completely, And shake and stir evenly to form a stable suspension as the water phase; take 20 ml of hexadecane, add 1 ml of emulsifier cetyl polyethylene glycol, mix and shake evenly to form an oil phase; put the oil phase into a small beaker, put Put in the magnet, carry out magnetic stirring at 700 rpm, drop the water phase into the stirring oil phase with a straw, keep stirring for 2 minutes until it is uniformly suspended, and then turn on the ultraviolet lamp (wavelength: 355-375nm, energy: 250mW) in the Above the small beaker, continue to irradiate, and the curing time lasts for 20 minutes; take out the solidified suspension, use cyclohexane and ethanol mixed washing solution (V / V, 1:1) to wash several times by centrifugation, and suspend the remaining solid in ethanol , using a stainless st...

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Abstract

The invention discloses a preparation method of a coding microsphere. The preparation method comprises the following steps: (1) mixing a coding material with aqueous oligomer, adding a corresponding photoinitiator, completely dissolving the photoinitiator, vibrating and evenly stirring the solution to form a stable suspension liquid, and taking the suspension liquid as the water phase; (2) mixing an alkane solvent and an emulsifier, and evenly vibrating the mixture to form an oil phase; (3) dropwise adding the water phase into the oil phase under stirring, wherein the volume of the oil phase is 20 to 30 times larger than that of the water phase, after addition, keeping on stirring the mixture until a uniform and stable suspension system is formed, and continuously irradiating the suspension system by UV light; and (4) washing the cured product for several times, screening out particles with a target size, and drying the particles to obtain the coding microspheres. The prepared coding microsphere has good hydrophilicity and an excellent coding performance, so the coding microsphere can be easily applied to surface modification and probe grafting and has a stable decoding performance for a liquid phase biochip.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and molecular biology, and relates to the preparation of a liquid-phase biochip carrier coded microsphere with a biomolecular recognition function, in particular to a preparation method of the coded microsphere. Background technique [0002] The liquid-phase biochip technology is to carry out the grafting of biological probes on the surface of the micro-nano-scale spherical support, and then carry out the hybridization reaction with the sample to be tested, detect the information of the target in the sample to be tested, and complete the analysis of the sample to be tested. Compared with traditional solid-based biochips, liquid-phase biochips have high detection sensitivity, less sample consumption, and high bioaffinity. The most important point is that using different probe microspheres for the hybridization reaction of the same sample to be tested can simultaneously complete the det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02C09K11/02
CPCB01J13/02C09K11/02
Inventor 何永红何清华孙树清
Owner 广州广华深启科技有限责任公司
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