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Aerobic culture and gas collection integrated indoor soil culture apparatus, and application thereof

A gas collection and culture device technology, applied in the research field, can solve problems such as the inability to quantify the initial culture concentration of the culture container, affect the gas production and discharge in the culture container, and affect the accuracy of the test, so as to achieve true and effective results of culture and detection, and low price , the effect of overcoming absorption problems

Active Publication Date: 2017-07-11
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the device cannot quantify the initial culture concentration in the culture vessel, and the injection of air during the culture period will also affect the gas production and discharge in the culture vessel; in addition, the use of fine needles inserted into the silica gel plug to obtain air may easily cause needle blockage and long-term use of silica gel. Air plugging affects the accuracy of the test, which is also a common problem in the prior art
[0006] After searching, there is no publication of relevant literature on an indoor soil cultivation device that integrates aeration cultivation and gas collection that is more realistic, effective and closer to reality.

Method used

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  • Aerobic culture and gas collection integrated indoor soil culture apparatus, and application thereof
  • Aerobic culture and gas collection integrated indoor soil culture apparatus, and application thereof
  • Aerobic culture and gas collection integrated indoor soil culture apparatus, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1. An indoor soil cultivation device integrating ventilation cultivation and gas collection

[0056] An indoor soil cultivation device integrating aeration cultivation and gas collection, the structure is as follows: figure 1 , 2 , 3, including the culture container 9, the top opening of the culture container 9 is fixedly provided with a partition 8 matching the top opening, the partition 8 includes a partition body and a gas opening and closing device, and the top opening is airtightly and fixedly connected on the partition The ventilation container 6 is provided with a vent 10 on the side wall of the ventilation container 6, and a top cover 5 is buckled on the ventilation container, and the top cover 5 is provided with an air inlet 4 and an air sampling hole 17, and an air intake pipe is fixed in the air inlet 4 2. One end of the air intake pipe 2 is inserted into the culture container, and the other end is connected with an air intake three-way valve 3. T...

Embodiment 2

[0063] A method utilizing the device of embodiment 1 to carry out soil cultivation and gas collection, comprising soil aeration cultivation, airtight cultivation, gas collection, the steps are as follows:

[0064] (1) Soil aeration culture

[0065] Unplug the top cover, put the prepared soil sample into the culture container, cover the top cover, open the gas opening and closing device, lift the top cover upwards, expose the vent on the side wall of the ventilation container, and adjust the gas collection tee at the same time Valve and air intake three-way valve to realize free ventilation inside and outside the culture container for aerobic culture. Weigh and record the total weight of the soil sample and the culture device before culture. During the culture process, use the gravimetric method to replenish the water lost due to evaporation every 2-4 days , Use a syringe with a long and thin needle to supplement distilled water into the culture container through a three-way va...

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Abstract

The invention relates to an aerobic culture and gas collection integrated indoor soil culture apparatus, and an application thereof. The apparatus comprises a culture container, the top mouth of the culture container is provided with a separator plate matched with the top mouth, the separator plate comprises a separator plate body and a gas opening and closing device, the separator plate is in closed fixed connection with an aeration container with an open top end, the sidewall of the aeration container is provided with an aeration opening, the ventilation container is covered with a top cover, the top cover is provided with a gas inlet hole and a gas collecting hole, a gas inlet tube is fixed in the gas inlet hole, one end of the gas inlet tube is inserted into the culture container, the other end of the gas inlet tube is connected with a balancing tube, a gas collecting tube is fixed in the gas collecting hole, one end of the gas collecting tube is inserted into the culture container, and the other end of the gas collecting tube is connected with a gas collecting needle. The apparatus has the advantages of implementation of aerobic culture and gas collection on the apparatus, small size, use in a limited space, convenience in operation, realization of closed culture under normal pressure, quantification of the initial culture concentration of a target gas, solving of the problem of target gas absorption due to the negative pressure caused by sampling, and real and effective reality-approaching culture and detection result.

Description

technical field [0001] The invention relates to an indoor soil cultivation device integrating aeration cultivation and gas collection and its application, and belongs to the research technical field of soil simulation cultivation and greenhouse gas emission. Background technique [0002] Global warming has become the focus of worldwide attention, and its main driving factor is the enhancement of the greenhouse effect caused by the increase of greenhouse gas emissions in the atmosphere. carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O) are the three most important greenhouse gases in the atmosphere, and their contribution to global warming accounts for nearly 80%. It is estimated that 5%-20% of CO is in the atmosphere every year 2 , 15%-30% CH 4 and 80%-90% of N 2 O originates from the soil. Therefore, scholars at home and abroad have carried out relevant observations and research on the three types of greenhouse gas emissions in different ecosystem soi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24G01N1/28G01N1/22
CPCG01N1/2294G01N1/28G01N33/24
Inventor 徐钰江丽华刘兆辉张建军谭德水石璟杨岩魏建林崔荣宗
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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