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Skin external-use preparation composition comprising edelweiss plant cell culture extracts and preparation method of skin external-use preparation composition

A technology of alpine edelweiss and skin external preparations, applied in the direction of medical preparations containing active ingredients, drug combinations, skin care preparations, etc., can solve problems such as non-existence, achieve acne suppression, heal skin wounds, and protect the skin barrier Effect

Active Publication Date: 2017-07-07
BIO FD&C CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Recently, studies on the development of functional foods using plant resources and the use of plant resources as cosmetic materials are in vogue. However, for the research on edelweiss, there are only skin tissue regeneration and hair growth that contain edelweiss extract in the prior art. growth promoter (Patent Document 1), but studies on the use of plant cells cultured from alpine edelweiss, adventitious root cultures, or their extracts themselves as cosmetic materials do not yet exist

Method used

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  • Skin external-use preparation composition comprising edelweiss plant cell culture extracts and preparation method of skin external-use preparation composition
  • Skin external-use preparation composition comprising edelweiss plant cell culture extracts and preparation method of skin external-use preparation composition
  • Skin external-use preparation composition comprising edelweiss plant cell culture extracts and preparation method of skin external-use preparation composition

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Experimental program
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Effect test

Embodiment 1-1

[0106] Embodiment 1-1 is induced according to the plant cell of alpine edelweiss of the present invention

[0107] The edelweiss seeds used in the present invention (purchased by online shopping, place of origin: Holland) are soaked in 70% ethanol for 30 seconds, then washed with sterilized water, sterilized with disinfectant (30%ROX+Tween20) for 20 minutes , and washed 3 times with sterilized water. After the seeds germinated, cut the leaves of alpine edelweiss at once with a sharp knife, and put them into a mixture containing 6-benzylaminopurine 1mg / L, 2,4-D 0.3mg / L, 3% sucrose and agar 8g / L basic MS (Murashige and Skoog 1962, manufactured by Duchefa Company, Cat No.M0221) medium, and under the growth conditions of temperature 25 ℃, humidity 70%, carry out dark cultivation, Thus inducing primary plant cells. The pH of each medium was adjusted to 5.8 with 1 N sodium hydroxide (NaOH). The interval of subculture was set at 2 weeks ( figure 1 ).

Embodiment 1-2

[0108] Embodiment 1-2 according to the induction of alpine edelweiss plant body adventitious root of the present invention

[0109] In order to induce adventitious roots from plant cells of edelweiss, 30 g / L of sucrose, 2.0 g / L of opal (Gelite) and 0.5 mg / L of growth regulator substance IBA (indole-3-butyric acid) were added to MS basal medium. L, and then placed in a culture room at 25±2°C for 4 weeks of cultivation. Cut about 1 cm from the tip of the induced adventitious root, and inoculate it in a liquid medium prepared by adding 0.5 mg / L IBA (indole-3-butyric acid) and 50 g / L sucrose to 1 / 2 MS medium Inside. Then put them in a culture room at 25±2°C for 4 weeks of cultivation. The obtained adventitious roots were filtered with a stainless steel filter to separate the medium, and the water was fully removed with a clean paper towel, and then dried at 60° C. for 2 days before being used for the test. The pH of each medium was adjusted to 5.8 with 1 N sodium hydroxide (N...

Embodiment 1-3

[0110] Embodiment 1-3 according to the mass production of alpine edelweiss plant cell and adventitious root of the present invention

[0111] In order to mass-produce the alpine edelweiss plant cells and alpine edelweiss adventitious roots obtained through cultivation after aseptic induction from the alpine edelweiss plant body, as mentioned above, if the alpine edelweiss plant cells are produced in batches, the edelweiss plant cells containing 6-benzyl Aminopurine 1mg / L, 2,4-D 0.3mg / L MS medium is cultivated, if the adventitious root of alpine edelweiss is produced in batches, then in the medium containing 0.5mg / L IBA (indole-3-butyric acid), sucrose 50g / L of 1 / 2MS culture medium, and then put the above culture medium in a temperature of 25 ± 2 ° C in a culture room, and in a humidity of 70%, 20L balloon-type bioreactor (manufactured by Samsung Science Co., Ltd.) air supply The amount was about 0.1vvm and cultured for 5 weeks.

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Abstract

The invention relates to a skin external-use preparation composition with anti-inflammation and anti-ageing effect, comprising edelweiss plant cell culture extracts and a preparation method of the skin external-use preparation composition, in particular to a skin external-use preparation composition with the anti-inflammation and anti-ageing effect, comprising plant cell cultures and adventitious-root cultures induced from edelweiss fronds, or extracts of the cultures, and a preparation method of the skin external-use preparation composition. The skin external-use preparation composition comprising the edelweiss plant cell or adventitious-root cultures or the extracts of the cultures has the advantages of non-toxicity on skin cells and effects such as improving skin wrinkles, curing wounds, shrinking pores, inhibiting sebum secretion and improving acne.

Description

technical field [0001] The present invention relates to an anti-inflammation and anti-aging skin external preparation composition containing alpine edelweiss (Edelweiss, Leontopodium alpinum) plant cell culture extract and a manufacturing method thereof. Plant cell cultures of grass plants, adventitious root cultures or their extracts have anti-aging, whitening, wound healing, anti-inflammation, sebum suppression, acne suppression, skin protection from UV damage, skin barrier protection, etc. Composition for external use and method for producing the same. Background technique [0002] With the passage of time, the secretion of various hormones used to regulate the metabolism of the human body gradually decreases, and the function and cell activity of immune cells also gradually decrease, so that the biosynthesis of the immune proteins necessary for the human body and the proteins that make up the human body gradually decrease. Intrinsic aging caused by aging, and photoaging...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K8/9789A61P17/00A61P17/02A61P17/08A61P17/10A61Q19/00A61Q17/04A61Q19/02A61Q19/08
CPCA61K8/97A61K36/28A61Q17/04A61Q19/00A61Q19/02A61Q19/08
Inventor 牟相炫徐孝铉李政勋牟志洪金守润朴廷坤郑海秀李承俊申东宣
Owner BIO FD&C CO LTD
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