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A method for tissue culture and rapid propagation of traditional Chinese medicine red-backed silk

A red-backed silk tissue culture and rapid propagation technology, applied in the field of plant tissue culture, can solve the problems of no patent application for red-backed silk tissue culture and rapid propagation, no red-backed silk tissue culture technology, etc., and achieve low cost and easy operation The effect of strong performance and simple process

Inactive Publication Date: 2019-03-12
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of red-backed silk at home and abroad, and there is no application for a patent for tissue culture and rapid propagation of red-backed silk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In the field, select the middle and upper part of the plant of the bitter vine with no obvious diseases, and the sunny and substantial canes with joints as explants. Immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time. .

[0020] (2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 5 seconds, and rinsed with sterile water for 5 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 15 minutes, rinse it with sterile water for 5 times, dry it with sterile filter paper, cut it into 1.5-2.5cm canes with joints and cut them into Inoculate into the induction medium, and place it at 25° C. for 45 days in full dark culture to induce the formation of adventiti...

Embodiment 2

[0026] (1) Collection of explants: In the field, select the middle and upper part of the plant of the bitter vine with no obvious diseases, and the sunny and substantial canes with joints as explants. Immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time. .

[0027] (2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 7 seconds, and rinsed with sterile water for 6 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 17 minutes, rinse it with sterile water for 6 times, dry it with sterile filter paper, cut it into 1.5-2.5cm canes with joints and cut them into Inoculate it into the induction medium and place it at 27°C for 47 days in total darkness to induce the formation of adventitious...

Embodiment 3

[0033] (1) Collection of explants: In the field, select the middle and upper part of the plant of the bitter vine with no obvious diseases, and the sunny and substantial canes with joints as explants. Immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time. .

[0034](2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 10 seconds, and rinsed with sterile water for 7 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 20 minutes, rinse it with sterile water for 7 times, dry it with sterile filter paper, cut it into 1.5-2.5cm canes with joints and cut them into Inoculate into the induction medium and place it at 28°C for 50 days in total darkness to induce the formation of adventitious bu...

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PUM

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Abstract

The invention relates to plant tissue culture methods of agricultural biotechnologies, specifically to a tissue culture and rapid propagation method of traditional Chinese medicine cissus assamica. The method includes the following steps in order: acquisition of a cissus assamica explant, induction culture of the explant, subculture, rooting culture and transplantation. An explant induction medium involved in the explant induction culture operation includes: an MS medium, 2.0-4.0mg / L 6-BA, 0.5-1.0mg / L NAA, 20-30g / L sucrose, 3.5-4.0g / L agar, 60-100ml / L coconut milk, and 0.1-0.3g / L activated carbon, and the pH of the explant induction medium is 5.4-5.8. The method provided by the invention selects a cissus assamica stem-segment with node as the explants, carries out induction culture, subculture, rooting culture, transplantation and other steps, reaches an induction rate of 88% or above and a survival rate of 94% or above, and realizes tissue culture and rapid propagation of traditional Chinese medicine cissus assamica, thus realizing the purpose of the invention.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of traditional Chinese medicine red-backed silk. Background technique [0002] Chinese medicine red back silk, that is Cissus assamica (Laws.) Craib., also known as Cissus assamica (Laws.) Craib. It has blood stasis and pain relief effects. It is commonly used in the folk for the treatment of bruises, rheumatic joint pain, carbuncle, swollen poison, snake bites and other diseases, especially poisonous snake bites. Studies have found that red back silk has the effect of antagonizing endothelin, and its ethanol The extract or ethyl acetate extract or the resveratrol isolated therefrom had obvious antagonistic effects on ET-1 at the whole and in vitro levels, and significantly antagonized the induced by ET-1 in isolated rat aortic strips. The contraction is in a dose-dependent manner, and it can anta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁钧淞莫昭展杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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