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A method for tissue culture and rapid propagation of apple rootstock mm116

A technology of MM116, tissue culture and rapid propagation, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of limited seedlings, dwarf rootstock breeding technology has not yet achieved breakthroughs, lack of adaptability and excellent dwarf rootstocks, etc., Achieve high survival rate, retain excellent properties, convenient methods and approaches

Inactive Publication Date: 2016-03-02
NORTHWEST A & F UNIV
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Problems solved by technology

At present, the dwarf apple seedling market in my country is in short supply. The key to restricting the development of the seedling industry lies in the lack of adaptable and excellent dwarf rootstocks on the one hand, and the lack of breakthroughs in the existing dwarf rootstock breeding technology on the other hand.

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  • A method for tissue culture and rapid propagation of apple rootstock mm116
  • A method for tissue culture and rapid propagation of apple rootstock mm116
  • A method for tissue culture and rapid propagation of apple rootstock mm116

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Embodiment Construction

[0013] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0014] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0015] Such as figure 1 As shown, the method for tissue culture and rapid propagation of apple rootstock MM116 of the embodiment of the present invention comprises the following steps:

[0016] S101: Using the apple rootstock MM116 as the material, choose a mother tree that grows vigorously and has no pests and diseases. Take 1-year-old branches in March and place them in a greenhouse at 25°C for hydroponics. Each liter of water contains 30g of sucrose. Change t...

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Abstract

The invention discloses an apple stock MM116 tissue culture rapid propagation method. The method comprises the steps as follows: firstly, conducting primary culture through adopting a culture medium containing MS+6-BA 0.5 mg / L+IBA 0.1 mg+PVP 500mg / L; secondly, conducting subculture by using MS+6-BA 0.6 mg / L+IBA 0.1 mg / L; thirdly, adopting a rooting culture medium of 1 / 2 MS+IBA 0.9 mg / L for rooting culture; fourthly, adopting pure saw dust for acclimatization and transplant. Through the method, the apple stock MM116 tissue culture rapid propagation is realized, a higher survival rate is guaranteed, the excellent characteristics of a stock is maintained, and a convenient method and approach are brought to the development and further expansion of the apple industry.

Description

technical field [0001] The invention belongs to the technical field of apple seedling cultivation, in particular to a method for tissue culture and rapid propagation of apple rootstock MM116. Background technique [0002] The apple industry occupies a very important position in China's agricultural economy. In 2013, China's apple planting area was 2.253 million hectares, with an output of 31.7 million tons, accounting for 1 / 2 of the world's total output. Compared with the world's advanced apple producing countries, there is still a big gap in my country's apple production, which is mainly manifested in low yield per unit area, poor fruit quality, low economic benefits, and a small share in the international fresh fruit market. The status of big countries is extremely disproportionate, and the poor quality of apple seedlings is one of the important reasons for this situation. At present, my country's apple production mode has gradually changed from the traditional low-efficie...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张东韩明玉王超李有梅韩静赵彩平
Owner NORTHWEST A & F UNIV
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