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A method for tissue culture and rapid propagation of lemongrass

A technology for tissue culture rapid propagation and citronella, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of easy accumulation and spread of viruses, inability to meet the needs of production and markets, and low reproduction efficiency. Achieve the effect of high multiplication coefficient, shortening the seedling cycle, easy and fast propagation

Active Publication Date: 2019-02-01
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lemongrass is generally propagated by branching, but the reproduction efficiency of this method is very low and cannot meet the needs of production and the market. Moreover, long-term asexual reproduction is easy to accumulate and spread viruses

Method used

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  • A method for tissue culture and rapid propagation of lemongrass
  • A method for tissue culture and rapid propagation of lemongrass
  • A method for tissue culture and rapid propagation of lemongrass

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for rapid propagation of Lemongrass tissue culture, the main steps are as follows:

[0031] (1) Selection and treatment of explants: Select robust citronella grass on a sunny day, cut off the lower residual root system and upper leaves, keep the stem and part of the leaf sheath, keep the length of the stem at 2.5cm~3cm, and peel off the outer 1~3 layers The leaf sheaths were used as explant materials; the soil on the surface of the explants was rinsed off with water, soaked in a dephosphorylation detergent solution for 2 minutes, and at the same time, the explants were scrubbed with a soft brush, and then rinsed with tap water; Above, soak the explants in an alcohol solution with a volume concentration of 75% for 10 s, rinse with sterile water for 3 to 5 times, and then put them in a volume concentration of 0.1% HgCl 2 Soak in the solution for 8 minutes, rinse 3 times with sterile water;

[0032] (2) First-generation induction: Peel off 1-2 layers of leaf shea...

Embodiment 2

[0038] A method for rapid propagation of Lemongrass tissue culture, the main steps are as follows:

[0039] (1) Selection and treatment of explants: Select robust citronella grass on a sunny day, cut off the lower residual root system and upper leaves, keep the stem and part of the leaf sheath, keep the length of the stem at 2.5cm~3cm, and peel off the outer 1~3 layers The leaf sheaths were used as explant materials; the soil on the surface of the explants was rinsed off with water, soaked in a dephosphorylation detergent solution for 2 minutes, and at the same time, the explants were scrubbed with a soft brush, and then rinsed with tap water; Above, soak the explants in alcohol solution with volume concentration of 75% for 10 s, rinse with sterile water for 3 to 5 times, and then put them in 0.1% HgCl 2 Soak in the solution for 10 min, rinse with sterile water 4 times;

[0040] (2) First-generation induction: Peel off 1-2 layers of leaf sheaths from the explants obtained in ...

Embodiment 3

[0046] A method for rapid propagation of Lemongrass tissue culture, the main steps are as follows:

[0047] (1) Selection and treatment of explants: Select robust citronella grass on a sunny day, cut off the lower residual root system and upper leaves, keep the stem and part of the leaf sheath, keep the length of the stem at 2.5cm~3cm, and peel off the outer 1~3 layers The leaf sheaths were used as explant materials; the soil on the surface of the explants was rinsed off with water, soaked in a dephosphorylation detergent solution for 2 minutes, and at the same time, the explants were scrubbed with a soft brush, and then rinsed with tap water; Above, soak the explants in alcohol solution with volume concentration of 75% for 10 s, rinse with sterile water for 3 to 5 times, and then put them in 0.1% HgCl 2 Soak in the solution for 12 min, rinse with sterile water 5 times;

[0048] (2) First-generation induction: Peel off 1-2 layers of leaf sheaths from the explants obtained in ...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of Lemongrass, which includes explant selection and treatment, primary induction, subculture, rooting medium and seedling hardening and transplanting procedures; pruning robust Lemongrass as explants, The explants are sterilized and inoculated in the primary induction medium to obtain initial buds, and then the initial buds are inoculated in the subculture medium for 15 to 20 days of subculture to form clustered buds, and single buds with a height ≥ 3cm After cutting off the upper leaves, transfer them to rooting medium to induce rooting, and finally carry out hardening and transplanting to obtain living seedlings of Lemongrass. The present invention establishes the tissue culture and rapid propagation technology system of Lemongrass by sterilizing the explants of Lemongrass, induction of the first generation, subculture, rooting culture and other factors and cultivation methods, and adopting suitable seedling hardening and seedling raising measures. , the seedling traits are stable, which provides technical support for the industrial seedling cultivation of citronella tissue culture, and is of great significance for promoting the development of citronella industry.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of citronella grass. Background technique [0002] Lemongrass ( Cymbopogon citratus) belongs to Poaceae, a perennial herb native to subtropical regions. Its stems and leaves contain citral, which has a lemon fragrance and can extract citronella oil. Citronella oil has medical effects such as disinfection, sterilization, relieving asthma, relieving cough, and treating neuralgia and muscle pain. It is widely used in daily chemical and pharmaceutical industries. In addition, lemongrass has been developed as tea and seasoning in recent years, which can improve digestive function, invigorate the stomach and eliminate fat. At the same time, lemongrass has ornamental value and can be used for greening and fragrant homes. It is easy to cultivate, extensively managed, and water-retaining. With good performance,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 覃玉凤肖玉菲周丽珠陈晓明潘晓芳覃子海陈博雯刘雄盛张烨张晓宁刘海龙
Owner GUANGXI FORESTRY RES INST
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